Application Data
(The FITC rabbit monoclonal antibody (AAA282961) are tested in Western blot against FITC Goat IgG (AS001),TRITC Goat IgG (AS026) and Goat IgG (AS071) at 1:4000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Rabbit anti-Human FITC/5-FAM/6-FAM Monoclonal Antibody | anti-FITC/5-FAM/6-FAM antibody
FITC/5-FAM/6-FAM Rabbit mAb
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
FITC/5-FAM/6-FAM, Antibody; FITC/5-FAM/6-FAM Rabbit mAb; anti-FITC/5-FAM/6-FAM antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
FITC conjugated to KLH
Applicable Applications for anti-FITC/5-FAM/6-FAM antibody
ELISA, WB (Western Blot)
Cross Reactivity
Species independent
Immunogen
FITC
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Application Data
(The FITC rabbit monoclonal antibody (AAA282961) are tested in Western blot against FITC Goat IgG (AS001),TRITC Goat IgG (AS026) and Goat IgG (AS071) at 1:4000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Application Data
(The FITC rabbit monoclonal antibody (AAA282961) are tested in Western blot against FITC Goat IgG (AS001),TRITC Goat IgG (AS026) and Goat IgG (AS071) at 1:4000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Application Data
(Immobilized biotin-6 Fam, biotin-5 Fam, biotin-FITC, at 1 ug/mL (25 uL/well), of which EC<sub>50</sub> ranges are 0.18-0.40 ng/mL, 0.19-0.29 ng/mL and 0.15-0.29 ng/mL ,can bind anti 6-Fam/ 5-Fam/ FITC mAb (AAA282961), respectively.)
Application Data
(Immobilized biotin-6 Fam, biotin-5 Fam, biotin-FITC, at 1 ug/mL (25 uL/well), of which EC<sub>50</sub> ranges are 0.18-0.40 ng/mL, 0.19-0.29 ng/mL and 0.15-0.29 ng/mL ,can bind anti 6-Fam/ 5-Fam/ FITC mAb (AAA282961), respectively.)
Related Product Information for anti-FITC/5-FAM/6-FAM antibody
Fluorescein isothiocyanate (FITC) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm. The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules.
Product Categories/Family for anti-FITC/5-FAM/6-FAM antibody
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Product Notes
The FITC/5-FAM/6-FAM (Catalog #AAA282961) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FITC/5-FAM/6-FAM Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FITC/5-FAM/6-FAM can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FITC/5-FAM/6-FAM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FITC conjugated to KLH. It is sometimes possible for the material contained within the vial of "FITC/5-FAM/6-FAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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