ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using FKBP10 Rabbit mAb (AAA283046, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
Rabbit anti-Human FKBP10 Monoclonal Antibody | anti-FKBP10 antibody
FKBP10 Rabbit mAb
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
FKBP10, Antibody; FKBP10 Rabbit mAb; FKBP10; BRKS1; FKBP65; OI11; OI6; PPIASE; hFKBP65; FK506 binding protein 10; anti-FKBP10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
FDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYGENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSHDYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLPTGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDGKITVDELKLKSDEDEERVHEEL
Applicable Applications for anti-FKBP10 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 303-582 of human FKBP10 (NP_068758.3).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using FKBP10 Rabbit mAb (AAA283046, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using FKBP10 Rabbit mAb (AAA283046, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.)
WB (Western Blot)
(Western blot analysis of various lysates using FKBP10 Rabbit mAb (AAA283046) at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): A-431Exposure time: 15s.)
WB (Western Blot)
(Western blot analysis of various lysates using FKBP10 Rabbit mAb (AAA283046) at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): A-431Exposure time: 15s.)
Related Product Information for anti-FKBP10 antibody
The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined.
Product Categories/Family for anti-FKBP10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 39kDa/64kDa
Observed MW: 70kDa
Observed MW: 70kDa
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase FKBP10
UniProt Gene Name
FKBP10
UniProt Synonym Gene Names
FKBP65; PSEC0056; PPIase FKBP10; 65 kDa FKBP; FKBP-65; FKBP-10
UniProt Entry Name
FKB10_HUMAN
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Product Notes
The FKBP10 fkbp10 (Catalog #AAA283046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FKBP10 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FKBP10 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the FKBP10 fkbp10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FDSSYSRNHT YNTYIGQGYI IPGMDQGLQG ACMGERRRIT IPPHLAYGEN GTGDKIPGSA VLIFNVHVID FHNPADVVEI RTLSRPSETC NETTKLGDFV RYHYNCSLLD GTQLFTSHDY GAPQEATLGA NKVIEGLDTG LQGMCVGERR QLIVPPHLAH GESGARGVPG SAVLLFEVEL VSREDGLPTG YLFVWHKDPP ANLFEDMDLN KDGEVPPEEF STFIKAQVSE GKGRLMPGQD PEKTIGDMFQ NQDRNQDGKI TVDELKLKSD EDEERVHEEL. It is sometimes possible for the material contained within the vial of "FKBP10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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