IF (Immunofluorescence)
(ICC/IF analysis of FKBP4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FKBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human FkBP4 Monoclonal Antibody | anti-FkBP4 antibody
FkBP4 antibody
Gene Names
FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
FkBP4, Antibody; FkBP4 antibody; FK506 binding protein 4; FKBP52; FKBP59; HBI; Hsp56; p52; PPIase; FK506 binding protein 4 52 kDa FK506 binding protein; FKBP 4; FKBP 52; FKBP 59; FKBP4; FKBP52 protein; Hsp 56; HSP binding immunophilin; p59 protein; Rotamase; Peptidyl prolyl cis trans isomerase; Peptidylprolyl cis trans isomerase; T cell FK506 binding protein 59kD.; anti-FkBP4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT4D3
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
459
Applicable Applications for anti-FkBP4 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human FkBP4 (1-459aa) purified from E. coli
Clone Note
Anti-human FkBP4 mAb, clone AT4D3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FkBP4 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of FKBP4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FKBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of FKBP4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FKBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IHC (Immunohiostchemistry)
(Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
IHC (Immunohiostchemistry)
(Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : K562 cell lysate Lane 3. : Jurkat cell lysate Lane 4. : MCF-7 cell lysate)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : K562 cell lysate Lane 3. : Jurkat cell lysate Lane 4. : MCF-7 cell lysate)
Related Product Information for anti-FkBP4 antibody
FkBP4, also known as FkBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes.
Product Categories/Family for anti-FkBP4 antibody
References
Ostrow KL, et al., (2009) Clin Cancer Res. 15 (4):1184-91; Ma D, et al., (2008) J Biol Chem. 283 (38):25963-70
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,805 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase FKBP4
NCBI Official Synonym Full Names
FK506 binding protein 4
NCBI Official Symbol
FKBP4
NCBI Official Synonym Symbols
HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase FKBP4
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase FKBP4
UniProt Gene Name
FKBP4
UniProt Synonym Gene Names
FKBP52; PPIase FKBP4; FKBP51; 52 kDa FKBP; FKBP-52; p59; FKBP-4; HBI
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Product Notes
The FkBP4 fkbp4 (Catalog #AAA48049) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FkBP4 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FkBP4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the FkBP4 fkbp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FkBP4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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