WB (Western Blot)
(Anti-FNDC5 Antibody at 1:2000 dilution + human skeletal muscle lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse anti-Human FNDC5 Monoclonal Antibody | anti-FNDC5 antibody
FNDC5 Antibody
Gene Names
FNDC5; FRCP2; irisin
Reactivity
Human
Applications
ELISA, Western Blot
Synonyms
FNDC5, Antibody; FNDC5 Antibody; Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin, FNDC5, FRCP2; anti-FNDC5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
This FNDC5 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 12-152 amino acids from human FNDC5.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
212
Applicable Applications for anti-FNDC5 antibody
ELISA, WB (Western Blot)
Cellular Location
Cell membrane; Single-pass type I membrane protein Peroxisome membrane; Single-pass type I membrane protein. Note: Imported in peroxisomes through the PEX5 receptor pathway.
Tissue Location
Widely expressed, with highest levels in heart. Very low expression, if any, in colon, pancreas and spleen
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-FNDC5 Antibody at 1:2000 dilution + human skeletal muscle lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-FNDC5 Antibody at 1:2000 dilution + human skeletal muscle lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-FNDC5 antibody
Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.
Product Categories/Family for anti-FNDC5 antibody
References
Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Bechtel S.,et al.BMC Genomics 8:399-399(2007). Bostrom P.,et al.Nature 481:463-468(2012).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated: 23659
NCBI Official Full Name
Fibronectin type III domain-containing protein 5
NCBI Official Synonym Full Names
fibronectin type III domain containing 5
NCBI Official Symbol
FNDC5
NCBI Official Synonym Symbols
FRCP2; irisin
NCBI Protein Information
fibronectin type III domain-containing protein 5
UniProt Protein Name
Fibronectin type III domain-containing protein 5
UniProt Gene Name
FNDC5
UniProt Synonym Gene Names
FRCP2
UniProt Entry Name
FNDC5_HUMAN
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Product Notes
The FNDC5 fndc5 (Catalog #AAA290742) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FNDC5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FNDC5 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FNDC5 fndc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FNDC5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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