IF (Immunofluorescence)
(Immunofluorescent analysis of Focal Adhesion Kinase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Mouse anti-Human Focal Adhesion Kinase Monoclonal Antibody | anti-PTK2 antibody
Anti-Focal Adhesion Kinase Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
Focal Adhesion Kinase, Antibody; Anti-Focal Adhesion Kinase Antibody; anti-PTK2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of Focal Adhesion Kinase protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH7.3.
Applicable Applications for anti-PTK2 antibody
IP (Immunoprecipitation), IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified recombinant human FAK protein fragments expressed in E coli.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of Focal Adhesion Kinase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Focal Adhesion Kinase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Western blot analysis of Focal Adhesion Kinase expression in COS7 (A), HUVEC (B), A549 (C), Hela (D) whole cell lysates. (Predicted band size: 119; 99; 63; 48; 120; 39; 114 kD; Observed band size: 125 kD))
WB (Western Blot)
(Western blot analysis of Focal Adhesion Kinase expression in COS7 (A), HUVEC (B), A549 (C), Hela (D) whole cell lysates. (Predicted band size: 119; 99; 63; 48; 120; 39; 114 kD; Observed band size: 125 kD))
Related Product Information for anti-PTK2 antibody
Mouse monoclonal antibody to Focal Adhesion Kinase
Product Categories/Family for anti-PTK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119,233 Da
NCBI Official Full Name
focal adhesion kinase 1 isoform c
NCBI Official Synonym Full Names
protein tyrosine kinase 2
NCBI Official Symbol
PTK2
NCBI Official Synonym Symbols
FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
NCBI Protein Information
focal adhesion kinase 1; FADK 1; PTK2 protein tyrosine kinase 2; FAK-related non-kinase polypeptide; focal adhesion kinase-related nonkinase; protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71
UniProt Protein Name
Focal adhesion kinase 1
UniProt Gene Name
PTK2
UniProt Synonym Gene Names
FAK; FAK1; FADK 1; FRNK; PPP1R71
UniProt Entry Name
FAK1_HUMAN
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Product Notes
The PTK2 ptk2 (Catalog #AAA265783) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Focal Adhesion Kinase Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Focal Adhesion Kinase can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PTK2 ptk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Focal Adhesion Kinase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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