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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Fodrin Mouse Monoclonal Antibody (SPTAN1/3351). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human Fodrin/Alpha Spectrin II (SPTAN1)/NEAS Monoclonal Antibody | anti-SPTAN1 antibody
Fodrin/Alpha Spectrin II (SPTAN1)/NEAS
Gene Names
SPTAN1; NEAS; EIEE5; SPTA2
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Fodrin/Alpha Spectrin II (SPTAN1)/NEAS, Antibody; Fodrin/Alpha Spectrin II (SPTAN1)/NEAS; Alpha-II spectrin; brain; EIEE5; Fodrin alpha chain; NEAS; Non erythrocytic spectrin alpha; non-erythroid alpha chain; SPECA; Spectrin alpha chain brain; Spectrin; alpha; non-erythrocytic 1 (alpha-fodrin); Spna2; SPTA2; anti-SPTAN1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
SPTAN1/3351
Specificity
Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous and chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Spectrin I and spectrin I are present in erythrocytes, whereas spectrin II (also designated fodrin) and spectrin II (also designated fodrin) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Activation of calpain results in the breakdown of spectrin II, a neuronal cytoskeleton protein.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
2477
Applicable Applications for anti-SPTAN1 antibody
IHC (Immunohistochemistry)
Positive Control
Human colon or kidney tissues (IHC).
Immunogen
Recombinant fragment of human SPTAN1 protein (around aa 2351-2475) (exact sequence is proprietary)
Cellular Localization
Cytoplasm
Hu-chromosome Location
9q34.11
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Fodrin Mouse Monoclonal Antibody (SPTAN1/3351). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Fodrin Mouse Monoclonal Antibody (SPTAN1/3351). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified Fodrin Mouse Monoclonal Antibody (SPTAN1/3351). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified Fodrin Mouse Monoclonal Antibody (SPTAN1/3351). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Fodrin Mouse Monoclonal Antibody (SPTAN1/3351).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Fodrin Mouse Monoclonal Antibody (SPTAN1/3351).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon stained with Fodrin Mouse Monoclonal Antibody (SPTAN1/3351).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon stained with Fodrin Mouse Monoclonal Antibody (SPTAN1/3351).)
References
Wang Y et al. Critical roles of aII spectrin in brain development and epileptic encephalopathy. J Clin Invest 128:760-773 (2018).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
240kDa
NCBI Official Full Name
spectrin alpha chain, non-erythrocytic 1 isoform 1
NCBI Official Synonym Full Names
spectrin alpha, non-erythrocytic 1
NCBI Official Symbol
SPTAN1
NCBI Official Synonym Symbols
NEAS; EIEE5; SPTA2
NCBI Protein Information
spectrin alpha chain, non-erythrocytic 1
UniProt Protein Name
Spectrin alpha chain, non-erythrocytic 1
UniProt Gene Name
SPTAN1
UniProt Synonym Gene Names
NEAS; SPTA2
UniProt Entry Name
SPTN1_HUMAN
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Product Notes
The SPTAN1 sptan1 (Catalog #AAA215207) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Fodrin/Alpha Spectrin II (SPTAN1)/NEAS reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fodrin/Alpha Spectrin II (SPTAN1)/NEAS can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SPTAN1 sptan1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fodrin/Alpha Spectrin II (SPTAN1)/NEAS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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