WB (Western Blot)
(Detection of human FOXO3a by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, LNCaP, Hep-G2, RKO, Jurkat, MCF-7, GaMG, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXO3a recombinant monoclonal antibody (AAA213601 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
FOXO3a recombinant antibody
Rabbit anti-FOXO3a Recombinant Monoclonal Antibody [BLR140H]
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
FOXO3a, Recombinant Antibody; Rabbit anti-FOXO3a Recombinant Monoclonal Antibody [BLR140H]; FKHRL1; forkhead homolog (rhabdomyosarcoma) like 1; FOXO3A; FOXO2; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; forkhead in rhabdomyosarcoma-like 1; forkhead box protein O3; FKHRL1P2; AF6q21 protein; AF6q21; forkhead box O3A; Forkhead box protein O3; forkhead box O3; FOXO3a recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR140H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
40ug/ml (varies by lot)
Applicable Applications for FOXO3a recombinant antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 475 and 525
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human FOXO3a by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, LNCaP, Hep-G2, RKO, Jurkat, MCF-7, GaMG, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXO3a recombinant monoclonal antibody (AAA213601 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human FOXO3a by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, LNCaP, Hep-G2, RKO, Jurkat, MCF-7, GaMG, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXO3a recombinant monoclonal antibody (AAA213601 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse FOXO3a by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXO3a recombinant monoclonal antibody (AAA213601 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse FOXO3a by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXO3a recombinant monoclonal antibody (AAA213601 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXO3a recombinant monoclonal antibody (AAA213601 lot 1) used for IP at 20 ul/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibodies and For blotting immunoprecipitated FOXO3a, AAA213601 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXO3a recombinant monoclonal antibody (AAA213601 lot 1) used for IP at 20 ul/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibodies and For blotting immunoprecipitated FOXO3a, AAA213601 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for FOXO3a recombinant antibody
Forkhead box protein 03A (FOXO3a) belongs to a large family of Forkhead proteins that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). FOXO3a is a transcription factor that functions to induce apoptosis in the absence of survival factors and in response to oxidative stress. Under conditions of oxidative stress FOXO3a is acetylated and localized to the nucleus where it may act as a transcriptional repressor.
Product Categories/Family for FOXO3a recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,277 Da
NCBI Official Full Name
forkhead box protein O3
NCBI Official Synonym Full Names
forkhead box O3
NCBI Official Symbol
FOXO3
NCBI Official Synonym Symbols
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
NCBI Protein Information
forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
UniProt Protein Name
Forkhead box protein O3
UniProt Gene Name
FOXO3
UniProt Synonym Gene Names
FKHRL1; FOXO3A
UniProt Entry Name
FOXO3_HUMAN
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Product Notes
The FOXO3a foxo3 (Catalog #AAA213601) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FOXO3a Recombinant Monoclonal Antibody [BLR140H] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FOXO3a can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FOXO3a foxo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXO3a, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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