FOXP1 recombinant antibody

Rabbit anti-FOXP1 Recombinant Monoclonal Antibody [BLR134H]

Cat. Num. AAA23845

• Gene Names: FOXP1; QRF1; 12CC4; hFKH1B; HSPC215; FLJ23741; MGC12942; MGC88572; MGC99551
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: FOXP1; Monoclonal Recombinant Antibody; Rabbit anti-FOXP1 Recombinant Monoclonal Antibody [BLR134H]; HSPC215; QRF1; mac-1-regulated forkhead; hFKH1B; glutamine-rich factor 1; forkhead box protein P1; fork head-related protein like B; 12CC4; MFH; Forkhead box protein P1; forkhead box P1; FOXP1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR134H
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 30ug/ml (varies by lot)

• Applicable Applications for FOXP1 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Between 627 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse FOXP1 by western blot. Samples: Whole cell lysate (25 ug) from mIMCD-3, A20, NIH 3T3, CT26, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXP1 recombinant monoclonal antibody (AAA23845 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of human FOXP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, A-549, HEK293T, MCF-7, THP-1, SK-N-BE(2), Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXP1 recombinant monoclonal antibody (AAA23845 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human FOXP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXP1 recombinant monoclonal antibody (AAA23845 lot 1) used for IP at 20 ul/mg lysate. FOXP1 was also immunoprecipitated by anti-FOXP1 antibodies BL-1894B-1A6 and A301-620A. For blotting immunoprecipitated FOXP1, AAA23845 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of mouse FOXP1 in FFPE renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-FOXP1 recombinant monoclonal antibody (AAA23845 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human FOXP1 in FFPE Burkitt lymphoma by immunohistochemistry. Antibody: Rabbit anti-FOXP1 recombinant monoclonal antibody (AAA23845 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human FOXP1 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-FOXP1 recombinant monoclonal antibody (AAA23845 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human FOXP1 in FFPE LNCaP cells by immunocytochemistry. Antibody: Rabbit anti-FOXP1 recombinant monoclonal antibody (AAA23845 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human FoxP1 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-FoxP1 recombinant monoclonal antibody (AAA23845 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for FOXP1 recombinant antibody

FOXP1 (forkhead box protein P1) is a member of the forkhead box (FOX) subfamily P family of proteins. FoxP1 functions as a transcriptional repressor and plays an important role in the specification and differentiation of lung epithelium. It can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Additionally, FOXP1 is an essential transcriptional regulator of B cell development [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H334].

Product Categories/Family for FOXP1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 18644886
• NCBI GeneID: 27086
• NCBI Accession #: NP_116071.2
• NCBI GenBank Nucleotide #: NM_032682.4
• UniProt Accession #: Q9H334; Q548T7; Q6IAJ1; Q8IXF4; Q8N2P0; Q8NAN6; Q8TEA2; Q9BSG9
• Molecular Weight: 52 kDa
• NCBI Official Full Name: forkhead box protein P1 isoform 1
• NCBI Official Synonym Full Names: forkhead box P1
• NCBI Official Symbol: FOXP1
• NCBI Official Synonym Symbols: QRF1; 12CC4; hFKH1B; HSPC215; FLJ23741; MGC12942; MGC88572; MGC99551
• NCBI Protein Information: forkhead box protein P1; OTTHUMP00000213499; OTTHUMP00000213500; OTTHUMP00000213501; OTTHUMP00000213502; OTTHUMP00000213503; OTTHUMP00000213504; OTTHUMP00000213506; OTTHUMP00000213507; OTTHUMP00000213508; glutamine-rich factor 1; fork head-related protein
• UniProt Protein Name: Forkhead box protein P1
• UniProt Gene Name: FOXP1
• UniProt Entry Name: FOXP1_HUMAN