WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Mouse GABA(A) Receptor Beta3 Monoclonal Antibody | anti-Gabrb3 antibody
GABA-A Receptor Beta3 Antibody: ATTO 390
Gene Names
Gabrb3; Cp1; beta3; Gabrb-3; AW049585; A230092K12Rik
Reactivity
Mouse, Human, Rat
Applications
Immunocytochemistry, Western Blot
Synonyms
GABA(A) Receptor Beta3, Antibody; GABA-A Receptor Beta3 Antibody: ATTO 390; Gabrb3; GABA(A) receptor subunit beta3; anti-Gabrb3 antibody
Host
Mouse
Reactivity
Mouse, Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S87-25
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
473
Applicable Applications for anti-Gabrb3 antibody
ICC (Immunocytochemistry), WB (Western Blot)
Conjugate
ATTO 390
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25. Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25. Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)
Related Product Information for anti-Gabrb3 antibody
Background Info: Detects ~55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta1.
Scientific Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).
Scientific Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).
Product Categories/Family for anti-Gabrb3 antibody
References
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 4. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,166 Da
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit beta-3 isoform a
NCBI Official Synonym Full Names
gamma-aminobutyric acid (GABA) A receptor, subunit beta 3
NCBI Official Symbol
Gabrb3
NCBI Official Synonym Symbols
Cp1; beta3; Gabrb-3; AW049585; A230092K12Rik
NCBI Protein Information
gamma-aminobutyric acid receptor subunit beta-3; cleft palate 1; GABA(A) receptor subunit beta-3; gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit beta-3
UniProt Gene Name
Gabrb3
UniProt Synonym Gene Names
Gabrb-3
UniProt Entry Name
GBRB3_MOUSE
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Product Notes
The Gabrb3 gabrb3 (Catalog #AAA103247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GABA-A Receptor Beta3 Antibody: ATTO 390 reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GABA(A) Receptor Beta3 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Gabrb3 gabrb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABA(A) Receptor Beta3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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