Mouse GAD2/GAD65 Monoclonal Antibody | anti-GAD2 antibody

GAD2/GAD65 (GABAergic Neuronal Marker)

Cat. Num. AAA214650

• Gene Names: GAD2; GAD65
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry, Western Blot, ELISA
• Synonyms: GAD2/GAD65, Antibody; GAD2/GAD65 (GABAergic Neuronal Marker); DCE2; GAD2; Glutamic Acid Decarboxylase 2 (Pancreas); Glutamic Acid Decarboxylase 2 (Pancreatic Islets and Brain 65kDa); Glutamic Acid Decarboxylase 65 (GAD65); anti-GAD2 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: GAD2/1960
• Specificity: This MAb recognizes a protein of 65kDa, which is identified as glutamic acid decarboxylase 2 (GDA2). It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD s) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67). GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 585

• Applicable Applications for anti-GAD2 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 10p12.1
• Positive Control: Pancreas or Brain.

Application Data

(Analysis of Protein Array containing more than 19, 000 full-length human proteins using GAD2 (GAD65) Mouse Monoclonal Antibody (GAD2/1960) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified GAD2 (GAD65) Mouse Monoclonal Antibody (GAD2/1960).Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Pancreas stained with GAD2 (GAD65) Mouse Monoclonal Antibody (GAD2/1960).)

References

Kaufman DL., et al. J Clin Invest. 1992 Jan; 89(1):283-92.

NCBI and Uniprot Product Information

• NCBI GI #: 4503875
• NCBI GeneID: 2572
• NCBI Accession #: NP_000809.1
• NCBI GenBank Nucleotide #: NM_000818.2
• UniProt Accession #: Q05329; Q9UD87
• Molecular Weight: 65kDa
• NCBI Official Full Name: glutamate decarboxylase 2
• NCBI Official Synonym Full Names: glutamate decarboxylase 2
• NCBI Official Symbol: GAD2
• NCBI Official Synonym Symbols: GAD65
• NCBI Protein Information: glutamate decarboxylase 2
• UniProt Protein Name: Glutamate decarboxylase 2
• UniProt Gene Name: GAD2
• UniProt Synonym Gene Names: GAD65; GAD-65