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Application Data
(The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Anti-GAPDH monoclonal antibody (1:20,000)Lane 2.: Anti-GAPDH monoclonal antibody (1:10,000)Lane 3.: Anti-GAPDH monoclonal antibody (1:2,000)Lane 4.: Anti-GAPDH monoclonal antibody (1:200))
Mouse anti-Human GAPDH Monoclonal Antibody | anti-GAPDH antibody
GAPDH antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
GAPDH, Antibody; GAPDH antibody; Glyceraldehyde-3-phosphate dehydrogenase isoform 2; G3PD; GAPD; HEL-S-162eP; anti-GAPDH antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT4E8
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration
0.2mg/ml (determined by BCA assay) (varies by lot)
Applicable Applications for anti-GAPDH antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
GAPDH from rabbit muscle
Conjugation
Unconjugated
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
Application Data
(The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Anti-GAPDH monoclonal antibody (1:20,000)Lane 2.: Anti-GAPDH monoclonal antibody (1:10,000)Lane 3.: Anti-GAPDH monoclonal antibody (1:2,000)Lane 4.: Anti-GAPDH monoclonal antibody (1:200))
Application Data
(The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Anti-GAPDH monoclonal antibody (1:20,000)Lane 2.: Anti-GAPDH monoclonal antibody (1:10,000)Lane 3.: Anti-GAPDH monoclonal antibody (1:2,000)Lane 4.: Anti-GAPDH monoclonal antibody (1:200))
Application Data
(The tissue lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:200). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse Brain tissue lysateLane 2.: Mouse Eye tissue lysateLane 3.: Mouse Muscle tissue lysate)
Application Data
(The tissue lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:200). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse Brain tissue lysateLane 2.: Mouse Eye tissue lysateLane 3.: Mouse Muscle tissue lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:200). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HepG2 cell lysateLane 2.: MCF7 cell lysateLane 3.: Jurkat cell lysateLane 4.: A549 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:200). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HepG2 cell lysateLane 2.: MCF7 cell lysateLane 3.: Jurkat cell lysateLane 4.: A549 cell lysate)
IF (Immunofluorescence)
(ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0592 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0592 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Related Product Information for anti-GAPDH antibody
Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis in the cytoplasm, it is involved in the translational control of gene expression in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.
Product Categories/Family for anti-GAPDH antibody
References
Chuang DM., et al, (2005) Annu Rev Pharmacol Toxicol. 45:269-90. Mazzola JL., et al, (2002) Neurotoxicology. 23(4-5):603-9. Sirover MA. (1997) J Cell Biochem. 66(2):133-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 37 kDa
Observed: 36 kDa
Observed: 36 kDa
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 1
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD; HEL-S-162eP
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; epididymis secretory sperm binding protein Li 162eP
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
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Product Notes
The GAPDH gapdh (Catalog #AAA48359) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GAPDH antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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