IF (Immunofluorescence)
(Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.)
Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody
GAPDH Monoclonal Antibody
Gene Names
gapdh; gapd; cb609; mg:bb02e05; wu:fb33a10; wu:ft80f05
Reactivity
Human, Rat, Bovine, Caprine, Guinea pig, Rabbit, Porcine,
Applications
Immunofluorescence, Western Blot
Purity
Protein A/G Purified
Synonyms
GAPDH, Antibody; GAPDH Monoclonal Antibody; aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component; anti-GAPDH antibody
Host
Mouse
Reactivity
Human, Rat, Bovine, Caprine, Guinea pig, Rabbit, Porcine,
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Protein A/G Purified
Form/Format
PBS with 0.1% sodium azide, 50% glycerol, pH 7.4
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-GAPDH antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant human GAPDH protein expressed by E Coli
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.)
IF (Immunofluorescence)
(Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.)
WB (Western Blot)
(Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000.Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate.Lysates/proteins at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000.Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate.Lysates/proteins at 20ug per lane.)
WB (Western Blot)
(Western Blot analysis of GAPDH in various cell lines at dilution of 1:5000.Lane1: DU145 whole cell lysate, Lane2: OS-RC-2 whole cell lysate, Lane3:T-47D whole cell Lysates, Lane4: HEC-1B whole cell lysate, Lane5: HepG2 whole cell lysate, Lane6: HEK-293 whole cell lysate,. Lysates/proteins at 20ug per lane.)
WB (Western Blot)
(Western Blot analysis of GAPDH in various cell lines at dilution of 1:5000.Lane1: DU145 whole cell lysate, Lane2: OS-RC-2 whole cell lysate, Lane3:T-47D whole cell Lysates, Lane4: HEC-1B whole cell lysate, Lane5: HepG2 whole cell lysate, Lane6: HEK-293 whole cell lysate,. Lysates/proteins at 20ug per lane.)
Related Product Information for anti-GAPDH antibody
GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.
Product Categories/Family for anti-GAPDH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,784 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
gapdh
NCBI Official Synonym Symbols
gapd; cb609; mg:bb02e05; wu:fb33a10; wu:ft80f05
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
gapdh
UniProt Synonym Gene Names
GAPDH
Similar Products
Product Notes
The GAPDH gapdh (Catalog #AAA178011) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GAPDH Monoclonal Antibody reacts with Human, Rat, Bovine, Caprine, Guinea pig, Rabbit, Porcine, and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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