Mouse GATA-3 Monoclonal Antibody | anti-GATA-3 antibody

GATA-3 (Breast and Urothelial Marker)

Cat. Num. AAA214746

• Gene Names: GATA3; HDR; HDRS
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: GATA-3, Antibody; GATA-3 (Breast and Urothelial Marker); GATA3; GATA binding protein-3; GATA-binding factor 3; HDR; HDRS; Transacting T-cell-specific transcription factor GATA-3; anti-GATA-3 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: GATA3/2446
• Specificity: GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas. Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 444

• Applicable Applications for anti-GATA-3 antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant fragment of human GATA3 protein (around aa 357-436) (exact sequence is proprietary)
• Positive Control: MCF-7 or T47D Cells. Breast or Bladder Carcinoma.
• Cellular Localization: Nuclear
• Chromosome Location: 10p14
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using GATA-3 Mouse Monoclonal Antibody (GATA3/2446). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2446).)

References

Higgins JP, et al. Am J Surg Pathol. 2007; 31:673-680.; Liu H, et al. Am J Clin Pathol. 2012; 138:57-64.

NCBI and Uniprot Product Information

• NCBI GI #: 50541959
• NCBI GeneID: 2625
• NCBI Accession #: NP_001002295.1
• NCBI GenBank Nucleotide #: NM_001002295.1
• UniProt Accession #: P23771; Q5VWG7; Q5VWG8; Q96J16
• Molecular Weight: 50kDa
• NCBI Official Full Name: trans-acting T-cell-specific transcription factor GATA-3 isoform 1
• NCBI Official Synonym Full Names: GATA binding protein 3
• NCBI Official Symbol: GATA3
• NCBI Official Synonym Symbols: HDR; HDRS
• NCBI Protein Information: trans-acting T-cell-specific transcription factor GATA-3
• UniProt Protein Name: Trans-acting T-cell-specific transcription factor GATA-3
• UniProt Gene Name: GATA3