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ChIP (Chromatin immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using GATA3 Rabbit mAb antibody (AAA282772) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
Rabbit anti-Human GATA3 Monoclonal Antibody | anti-GATA3 antibody
GATA3 Rabbit mAb
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, ELISA, Immunoprecipitation, Western Blot
Purity
Affinity purification
Synonyms
GATA3, Antibody; GATA3 Rabbit mAb; HDR; HDRS; GATA3; anti-GATA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSH
Applicable Applications for anti-GATA3 antibody
ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation), ELISA, IP (Immunoprecipitation), WB (Western Blot)
Cross Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GATA3 (P23771).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
ChIP (Chromatin immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using GATA3 Rabbit mAb antibody (AAA282772) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
ChIP (Chromatin immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using GATA3 Rabbit mAb antibody (AAA282772) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of Jurkat cells using 3 ug GATA3 antibody (AAA282772). Western blot was performed from the immunoprecipitate using GATA3 antibody (AAA282772) at a dilution of 1:500.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of Jurkat cells using 3 ug GATA3 antibody (AAA282772). Western blot was performed from the immunoprecipitate using GATA3 antibody (AAA282772) at a dilution of 1:500.)
WB (Western Blot)
(Western blot analysis of various lysates using GATA3 Rabbit mAb (AAA282772) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of various lysates using GATA3 Rabbit mAb (AAA282772) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (AAA282772). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (AAA282772). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (AAA282772). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (AAA282772). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.)
Related Product Information for anti-GATA3 antibody
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.
Product Categories/Family for anti-GATA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 48kDa
Observed MW: 48kDa
Observed MW: 48kDa
UniProt Protein Name
Trans-acting T-cell-specific transcription factor GATA-3
UniProt Gene Name
GATA3
UniProt Entry Name
GATA3_HUMAN
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Product Notes
The GATA3 gata3 (Catalog #AAA282772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GATA3 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA3 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation), ELISA, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GATA3 gata3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LSHSYMDAAQ YPLPEEVDVL FNIDGQGNHV PPYYGNSVRA TVQRYPPTHH GSQVCRPPLL HGSLPWLDGG KALGSHHTAS PWNLSPFSKT SIHHGSPGPL SVYPPASSSS LSGGHASPHL FTFPPTPPKD VSPDPSLSTP GSAGSARQDE KECLKYQVPL PDSMKLESSH. It is sometimes possible for the material contained within the vial of "GATA3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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