Mouse GC Monoclonal Antibody | anti-GBA antibody

GC Antibody (Center) (Ascites)

Cat. Num. AAA283471

• Gene Names: GBA; GCB; GBA1; GLUC
• Reactivity: Human; Predicted Reactivity: Bovine, Pig
• Applications: ELISA, Western Blot
• Synonyms: GC, Antibody; GC Antibody (Center) (Ascites); Glucosylceramidase; Acid beta-glucosidase; Alglucerase; Beta-glucocerebrosidase; Beta-GC; D-glucosyl-N-acylsphingosine glucohydrolase; Imiglucerase; GBA; GC; GLUC; anti-GBA antibody

• Host: Mouse
• Reactivity: Human; Predicted Reactivity: Bovine, Pig
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 660CT8.6.6.2
• Specificity: This GC antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 337-365 amino acids from the Central region of human GC.
• Form/Format: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Sequence Positions: 337-365
• Sequence Length: 536

• Applicable Applications for anti-GBA antibody: ELISA, WB (Western Blot)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(GC Antibody(Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the GC antibody detected the GC protein (arrow).)

Related Product Information for anti-GBA antibody

Function: Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:9201993, PubMed:24211208, PubMed:15916907). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation (PubMed:19279011). Also plays a role in cholesterol metabolism (PubMed:24211208, PubMed:26724485). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208, PubMed:26724485). The short chain saturated C8:0- GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (PubMed:24211208). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also hydrolyze cholesteryl- beta-D-glucoside to produce D-glucose and cholesterol (PubMed:24211208, PubMed:26724485)

Cellular Location: Lysosome membrane; Peripheral membrane protein; Lumenal side. Note=Interaction with saposin-C promotes membrane association (PubMed:10781797). Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 (PubMed:18022370)

Product Categories/Family for anti-GBA antibody

Cancer; Metabolism; Neuroscience; Signal transduction

References

Dos Santos, A.V., et al. Neurosci. Lett. 485(2):121-124(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jeong, S.Y., et al. Blood Cells Mol. Dis. (2010) In press :
Hu, F.Y., et al. Eur. J. Neurol. (2010) In press :
Velayati, A., et al. Curr Neurol Neurosci Rep 10(3):190-198(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 54607043
• NCBI GeneID: 2629
• NCBI Accession #: P04062; NP_001005741.1; NP_001005742.1
• NCBI GenBank Nucleotide #: NM_000157.3
• Molecular Weight: 59716
• NCBI Official Full Name: glucosylceramidase isoform 1
• NCBI Official Synonym Full Names: glucosidase, beta, acid
• NCBI Official Symbol: GBA
• NCBI Official Synonym Symbols: GCB; GBA1; GLUC
• NCBI Protein Information: glucosylceramidase
• UniProt Protein Name: Glucosylceramidase
• UniProt Gene Name: GBA
• UniProt Synonym Gene Names: GC; GLUC; Beta-GC
• UniProt Entry Name: GLCM_HUMAN