IF (Immunofluorescence)
(Immunofluorescence staining of GDF3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GDF3 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human GDF3 Monoclonal Antibody | anti-GDF3 antibody
Recombinant Anti-GDF3 Antibody, Rabbit Monoclonal
Gene Names
GDF3; KFS3; MCOP7; MCOPCB6
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A
Synonyms
GDF3, Antibody; Recombinant Anti-GDF3 Antibody, Rabbit Monoclonal; GDF-3 Antibody, Rabbit MAb; growth differentiation factor 3; Anti-KFS3 Antibody; Anti-MCOP7 Antibody; Anti-MCOPCB6 Antibody; anti-GDF3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
010
Specificity
Human GDF-3
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GDF3 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Antibody Type
Recombinant Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human GDF-3
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human GDF-3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of GDF3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GDF3 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of GDF3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GDF3 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human GDF3 in human breast carcinoma with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human GDF3 in human breast carcinoma with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human GDF3 in human testis with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human GDF3 in human testis with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-GDF3 antibody
The GDF3 gene plays a fundamental role in embryonic morphogenesis. GDF3 expression is significantly decreased in human breast cancer tissues, and reconstitution of GDF3 in breast cancer may be a potential therapeutic approach to inhibit aggressive growth of breast cancer.
Product Categories/Family for anti-GDF3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,387 Da
NCBI Official Full Name
growth/differentiation factor 3
NCBI Official Synonym Full Names
growth differentiation factor 3
NCBI Official Symbol
GDF3
NCBI Official Synonym Symbols
KFS3; MCOP7; MCOPCB6
NCBI Protein Information
growth/differentiation factor 3; GDF-3
UniProt Protein Name
Growth/differentiation factor 3
UniProt Gene Name
GDF3
UniProt Synonym Gene Names
GDF-3
UniProt Entry Name
GDF3_HUMAN
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Product Notes
The GDF3 gdf3 (Catalog #AAA254201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-GDF3 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GDF3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the GDF3 gdf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDF3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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