WB (Western Blot)
(Anti-GFP rabbit monoclonal antibody at 1:500 dilutionLane A: GFP transfected E.coli lysate (5ug)Lane B: GFP transfected 293 Cell lysate (30ug)Lane C: Non-transfected 293 Cells lysate (30ug)SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Rabbit GFP Monoclonal Antibody | anti-GFP antibody
Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal
Reactivity
Aequorea victoria
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A
Synonyms
GFP, Antibody; Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal; GFP Antibody, Rabbit MAb; anti-GFP antibody
Host
Rabbit
Reactivity
Aequorea victoria
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
208
Specificity
Aequorea victoria GFP Tag
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GFP antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Aequorea victoria GFP protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-GFP rabbit monoclonal antibody at 1:500 dilutionLane A: GFP transfected E.coli lysate (5ug)Lane B: GFP transfected 293 Cell lysate (30ug)Lane C: Non-transfected 293 Cells lysate (30ug)SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
WB (Western Blot)
(Anti-GFP rabbit monoclonal antibody at 1:500 dilutionLane A: GFP transfected E.coli lysate (5ug)Lane B: GFP transfected 293 Cell lysate (30ug)Lane C: Non-transfected 293 Cells lysate (30ug)SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
IP (Immunoprecipitation)
(Anti-GFP Tag was immunoprecipitated using:Lane A: GFP transfected E.coli cell lysate (0.5mg)Lane B: GFP transfected 293 cell lysate (0.5mg) Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilutionSecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.)
IP (Immunoprecipitation)
(Anti-GFP Tag was immunoprecipitated using:Lane A: GFP transfected E.coli cell lysate (0.5mg)Lane B: GFP transfected 293 cell lysate (0.5mg) Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilutionSecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.)
Related Product Information for anti-GFP antibody
Green fluorescent protein (GFP tag) is a ~27 kDa protein consisting of 238 amino acids derived from jellyfish Aequorea Victoria. GFP exhibits bright green fluorescent light when exposed to blue light. It has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. Anti GFP-tag antibodies are often used in biological research and related fields. GFP tag antibodies are also often been used to study the expression of proteins and the interaction between proteins and proteins.
References
Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
NCBI and Uniprot Product Information
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Product Notes
The GFP (Catalog #AAA255635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal reacts with Aequorea victoria and may cross-react with other species as described in the data sheet. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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