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WB (Western Blot)
(Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 ug) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody .)
GITR/TNFRSF18 recombinant antibody
Rabbit anti-GITR/TNFRSF18 Recombinant Monoclonal Antibody [BLR068G]
Gene Names
TNFRSF18; AITR; GITR; CD357; GITR-D
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
GITR/TNFRSF18, Recombinant Antibody; Rabbit anti-GITR/TNFRSF18 Recombinant Monoclonal Antibody [BLR068G]; GITR-D; tumor necrosis factor receptor superfamily member 18; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; GITR; CD357; CD antigen CD357; AITR; TNF receptor superfamily activation-inducible protein; Tumor necrosis factor receptor superfamily member 18; TNF receptor superfamily member 18; GITR/TNFRSF18 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR068G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for GITR/TNFRSF18 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed and permeabilized cells were stained with 0.2 ul per 1 x 10^6 cells.
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed and permeabilized cells were stained with 0.2 ul per 1 x 10^6 cells.
Immunogen
Between 191 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 ug) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 ug) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human GITR/TNFRSF18 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used for IP at 6 ul per reaction. GITR/TNFRSF18 was also immunoprecipitated by rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BL-1066-1D11]. For blotting immunoprecipitated GITR/TNFRSF18, AAA23817 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human GITR/TNFRSF18 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used for IP at 6 ul per reaction. GITR/TNFRSF18 was also immunoprecipitated by rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody [BL-1066-1D11]. For blotting immunoprecipitated GITR/TNFRSF18, AAA23817 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:200. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:200. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human GITR/TNFRSF18 by immunocytochemistry. Sample: FFPE section of RPMI-8226 cells. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human GITR/TNFRSF18 by immunocytochemistry. Sample: FFPE section of RPMI-8226 cells. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (AAA23817 lot 1) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human GITR/TNFRSF18 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal (AAA23817 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human GITR/TNFRSF18 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal (AAA23817 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for GITR/TNFRSF18 recombinant antibody
GITR/TNFRSF18 is a member of the TNF-receptor superfamily. It has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by regulatory T cells. GITR/TNFRSF18 also plays a role in the regulation of CD3-driven T-cell activation and programmed cell death. [provided by RefSeq, Feb 2011] [taken from NCBI Entrez Gene (Gene ID: 8784)].
Product Categories/Family for GITR/TNFRSF18 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,110 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 18 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily member 18
NCBI Official Symbol
TNFRSF18
NCBI Official Synonym Symbols
AITR; GITR; CD357; GITR-D
NCBI Protein Information
tumor necrosis factor receptor superfamily member 18
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 18
UniProt Gene Name
TNFRSF18
UniProt Synonym Gene Names
AITR; GITR
UniProt Entry Name
TNR18_HUMAN
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Product Notes
The GITR/TNFRSF18 tnfrsf18 (Catalog #AAA23817) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GITR/TNFRSF18 Recombinant Monoclonal Antibody [BLR068G] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GITR/TNFRSF18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 6 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed and permeabilized cells were stained with 0.2 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the GITR/TNFRSF18 tnfrsf18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GITR/TNFRSF18, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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