IHC (Immunohistochemistry)
(The human kidney tissue stained with Mouse GLEPP-1 monoclonal antibody (AAA14075) at 1:100 using AEC chromogen. (Note: formalin-fixed, paraffin-embedded sections need 15 minutes heat-induced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antiibody).)
Mouse GLEPP-1 Monoclonal Antibody | anti-GLEPP-1 antibody
Mouse anti GLEPP-1 Monoclonal Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
The Mouse IgG is purified by Protein A-Afinity Chromatography according to isotyping.
Synonyms
GLEPP-1, Antibody; Mouse anti GLEPP-1 Monoclonal Antibody; Glomerular Epithelial Protein 1; NPHS6; PTPU2; GLEPP1; PTP-OC; PTP-U2; PTPROT; R-PTP-O; anti-GLEPP-1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
5F3F8
Specificity
This antibody recognizes human GLEPP-1 protein. The other species are not tested.
Purity/Purification
The Mouse IgG is purified by Protein A-Afinity Chromatography according to isotyping.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH 7.2) containing antibody stabilizer.
Applicable Applications for anti-GLEPP-1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.1-1 ug/mL
ELISA: 0.01-0.1 ug/mL
Immunoprecipitation: 2-5 ug/mL
IHC: 2-10 ug/mL
Flow Cytometry: Not tested
Positive Control: Kidney Tissue
Cellular location: Cell Membrane
Optimal working dilutions must be determined by end user.
ELISA: 0.01-0.1 ug/mL
Immunoprecipitation: 2-5 ug/mL
IHC: 2-10 ug/mL
Flow Cytometry: Not tested
Positive Control: Kidney Tissue
Cellular location: Cell Membrane
Optimal working dilutions must be determined by end user.
Positive Control
Kidney
Cellular Location
Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C.
The antibodies can be stored at 2°C-8°C for one month without detectable loss of activity.
Avoid repeated freezing-thawing cycles.
The antibodies can be stored at 2°C-8°C for one month without detectable loss of activity.
Avoid repeated freezing-thawing cycles.
IHC (Immunohistochemistry)
(The human kidney tissue stained with Mouse GLEPP-1 monoclonal antibody (AAA14075) at 1:100 using AEC chromogen. (Note: formalin-fixed, paraffin-embedded sections need 15 minutes heat-induced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antiibody).)
IHC (Immunohistochemistry)
(The human kidney tissue stained with Mouse GLEPP-1 monoclonal antibody (AAA14075) at 1:100 using AEC chromogen. (Note: formalin-fixed, paraffin-embedded sections need 15 minutes heat-induced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antiibody).)
Related Product Information for anti-GLEPP-1 antibody
Glomerular Epithelial Protein 1 (GLEPP1) is a transmembrane protein tyrosine phosphatase found only in the epithelial cells of the renal glomerulus. It is localized to the apical surface of polarized cells and may have tissue-specific functions through activation of Src family kinases. It may have multiple isoform-specific and tissue-specific functions, including the regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis. This gene is a candidate tumor suppressor, and decreased expression of this gene has been observed in several types of cancer. GLEPP-1 is present only in visceral glomerular cells (Podocytes). The presence of GLEPP1 may be used as a marker of podocyte integrity in various forms of glomerular injury.
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Product Notes
The GLEPP-1 (Catalog #AAA14075) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti GLEPP-1 Monoclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLEPP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC), Immunoprecipitation (IP). Western blotting: 0.1-1 ug/mL ELISA: 0.01-0.1 ug/mL Immunoprecipitation: 2-5 ug/mL IHC: 2-10 ug/mL Flow Cytometry: Not tested Positive Control: Kidney Tissue Cellular location: Cell Membrane Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GLEPP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLEPP-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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