Mouse GLUTAMATE DECARBOXYLASE 2 Monoclonal Antibody | anti-GAD2 antibody

MOUSE ANTI HUMAN GLUTAMATE DECARBOXYLASE 2 (N-TERMINAL)

Cat. Num. AAA50182

• Gene Names: GAD2; GAD65
• Applications: Western Blot, Immunoprecipitation, ELISA, Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: GLUTAMATE DECARBOXYLASE 2, Antibody; MOUSE ANTI HUMAN GLUTAMATE DECARBOXYLASE 2 (N-TERMINAL); anti-GAD2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: N-GAD65
• Form/Format: Purified, Purified IgG - liquid; Phosphate buffered saline
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 585

• Applicable Applications for anti-GAD2 antibody: WB (Western Blot), IP (Immunoprecipitation), ELISA, IHC (Immunohistochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Immunogen: Keyhole Limpet Haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65.
• Histology Positive Control Tissue: Human brain
• Target Species: Human
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Application Data

(Human brain lysate probed with Mouse anti Human Glutamine Decarboxylase 2 followed by Goat anti Mouse IgG H+L:DyLight®800)

Related Product Information for anti-GAD2 antibody

Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. Glutamate decarboxylase 2 is a 585 amino acid ~65 kDa lipid anchored membrane associated protein responsible for catalysis of gamma -aminobutyric acid (GABA) production, the major inhibitory neurotransmitter in the central nervous system (Fenalti et al. 2007) and is expressed in the brain and pancreatic beta cells. Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease (Kallmann et al. 1997), Stiff Man Syndrome (Raju et al. 2005) and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes (Petersen et al. 1994). The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles (Shi et al. 1994). Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes GAD65 but does not recognize GAD67 (Hampe et al. 2001).

NCBI and Uniprot Product Information

• NCBI GI #: 4503875
• NCBI GeneID: 2572
• NCBI Accession #: NP_000809.1
• NCBI GenBank Nucleotide #: NM_000818.2
• UniProt Accession #: Q05329; Q9UD87
• NCBI Official Full Name: glutamate decarboxylase 2
• NCBI Official Synonym Full Names: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• NCBI Official Symbol: GAD2
• NCBI Official Synonym Symbols: GAD65
• NCBI Protein Information: glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform
• UniProt Protein Name: Glutamate decarboxylase 2
• UniProt Gene Name: GAD2
• UniProt Synonym Gene Names: GAD65; GAD-65
• UniProt Entry Name: DCE2_HUMAN