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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
Mouse anti-Human Glutathione Peroxidase 1 Monoclonal Antibody | anti-GPX1 antibody
Glutathione Peroxidase 1 Antibody
Gene Names
GPX1; GPXD; GSHPX1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
Glutathione Peroxidase 1, Antibody; Glutathione Peroxidase 1 Antibody; AL033363 antibody; Cellular glutathione peroxidase antibody; Glutathione peroxidase 1 antibody; Glutathione peroxidase antibody; GPx 1 antibody; GPx-1 antibody; GPX1 antibody; GPX1_HUMAN antibody; GPXD antibody; GSHPx-1 antibody; GSHPX1 antibody; MGC14399 antibody; MGC88245 antibody; anti-GPX1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
C5-F6
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-GPX1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin.)
Application Data
(Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution. Positive control: Lane 1: THP-1 Lane 2: HepG2 Lane 3: 293T)
Application Data
(Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution. Positive control: Lane 1: THP-1 Lane 2: HepG2 Lane 3: 293T)
Related Product Information for anti-GPX1 antibody
GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, gamma-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.
Product Categories/Family for anti-GPX1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,088 Da
NCBI Official Full Name
glutathione peroxidase 1 isoform 1
NCBI Official Synonym Full Names
glutathione peroxidase 1
NCBI Official Symbol
GPX1
NCBI Official Synonym Symbols
GPXD; GSHPX1
NCBI Protein Information
glutathione peroxidase 1; GPx-1; GSHPx-1; cellular glutathione peroxidase
UniProt Protein Name
Glutathione peroxidase 1
UniProt Gene Name
GPX1
UniProt Synonym Gene Names
GPx-1; GSHPx-1
UniProt Entry Name
GPX1_HUMAN
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Product Notes
The GPX1 gpx1 (Catalog #AAA312089) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Glutathione Peroxidase 1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione Peroxidase 1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GPX1 gpx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione Peroxidase 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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