Mouse anti-Human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody | anti-GAPDH antibody

Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Cat. Num. AAA21258

• Gene Names: GAPDH; G3PD; GAPD
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Protein A + Protein G affinity chromatography
• Synonyms: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH); Monoclonal Antibody; Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH); Glyceraldehyde-3-Phosphate Dehydrogenase; G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH; anti-GAPDH antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: F2
• Specificity: The antibody is a mouse monoclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.
• Purity/Purification: Protein A + Protein G affinity chromatography
• Form/Format: Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 1mg/mL (varies by lot)
• Sequence Positions: Gly2~Ser148

• Applicable Applications for anti-GAPDH antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 0.01-2ug/mL; IHC: 5-20ug/mL; ICC: 5-20ug/mL
• Immunogen: Recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
• Preparation and Storage: Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; Stable for 12 months if correctly stored.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Western Blot; Samples: Lane1: Gallus Kidney lysate; Lane2: Rabbit Liver lysate; Lane3: Rabbit Kidney lysate; Lane4: Ovine Liver lysate; Lane5: Mouse Liver lysate; Lane6: Rat Liver lysate; Lane7: Rat Kidney lysate;Primary Ab: 0.5ug/ml Mouse Anti-human GAPDH AntibodySecond Ab: 0.2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Sample: Cavia Liver lysatePrimary Ab: 0.5ug/ml Mouse Anti-human GAPDH AntibodySecond Ab: 0.2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Samples: Lane1: human Lung lysate; Lane2: 293T cell lysate; Lane3: Cavia Kidney lysate; Lane4: Porcine Liver lysate; Lane5: Porcine Kidney lysate;Primary Ab: 0.5ug/ml Mouse Anti-human GAPDH AntibodySecond Ab: 0.2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Samples: Lane1: Rat CerebuM lysate; Lane2: Hela cell lysate;Primary Ab: 1ug/ml Mouse Anti-human GAPDH AntibodySecond Ab: 0.2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Sample: Rat Heart lysate Primary Ab: 1ug/ml Mouse Anti-human GAPDH Antibody Second Ab: 0.2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: human liver Cirrhosis TissuePrimary Ab: 20ug/ml Mouse Anti-human GAPDH AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: human Lymphoma TissuePrimary Ab: 20ug/ml Mouse Anti-human GAPDH AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Sample: human Endometrial cancer Tissue Primary Ab: 20ug/ml Mouse Anti-human GAPDH Antibody Control: Used PBS instead of primary antibody Second Ab: 2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

Product Categories/Family for anti-GAPDH antibody

Monoclonal Antibodies (Primary Abs); Monoclonal antibodies; Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 378404908
• NCBI GeneID: 2597
• NCBI Accession #: NP_001243728.1
• NCBI GenBank Nucleotide #: NM_001256799.1
• UniProt Accession #: P04406; P00354; Q53X65
• Molecular Weight: 36,053 Da
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase isoform 2
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: GAPDH
• NCBI Official Synonym Symbols: G3PD; GAPD
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDH
• UniProt Synonym Gene Names: GAPD; GAPDH
• UniProt Entry Name: G3P_HUMAN