Mouse anti-Human GOLPH2/GOLM1 Monoclonal Antibody | anti-GOLM1 antibody

Anti-GOLPH2/GOLM1 Antibody, Mouse Monoclonal

Cat. Num. AAA257530

• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: Protein A
• Synonyms: GOLPH2/GOLM1, Antibody; Anti-GOLPH2/GOLM1 Antibody, Mouse Monoclonal; GOLPH2/GOLM1 Antibody, Mouse MAb; Anti-bA379P1.3 Antibody; Anti-C9orf155 Antibody; Anti-GOLPH2 Antibody; Anti-GP73 Antibody; Anti-HEL46 Antibody; Anti-PSEC0257 Antibody; golgi membrane protein 1; anti-GOLM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 12
• Specificity: Human GOLPH2/GOLM1
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-GOLM1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
• Immunogen: Recombinant Human GOLPH2/GOLM1 Protein
• Conjugation: Unconjugated
• Preparation: This product is a recombinant monoclonal antibody expressed from HEK293 cells.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IF (Immunofluorescence)

(Immunofluorescence staining of GP73 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human GP73 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to golgi apparatus.)

IHC (Immunohistochemisry)

(Immunochemical staining of human GP73 in human liver (cirrhosis) with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

IHC (Immunohiostchemistry)

(Immunochemical staining of human GP73 in human stomach with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry)

(Immunochemical staining of human GP73 in human uterus with mouse monoclonal antibody (8 ug/mL, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-GOLM1 antibody

Background: Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1/CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

Kladney R.D., et al.,(2000), GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53-65.Clark H.F., et al., (2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270.Puri S., et al.,(2002), Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.Traffic 3:641-653.