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WB (Western Blot)
(Detection of human GP130/CD130 by western blot. Samples: Whole cell lysate (50 ug) from NCI-H226, MCF-7, IMR-90, Jurkat, HeLa, A-549, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 .)
GP130/CD130 recombinant antibody
Rabbit anti-GP130/CD130 Recombinant Monoclonal Antibody [BLR114H]
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
Purified
Synonyms
GP130/CD130, Recombinant Antibody; Rabbit anti-GP130/CD130 Recombinant Monoclonal Antibody [BLR114H]; membrane glycoprotein gp130; IL-6R-beta; interleukin receptor beta chain; interleukin-6 receptor subunit beta; oncostatin-M receptor subunit alpha; Interleukin-6 signal transducer; membrane glycoprotein 130; IL-6RB; CD130 antigen; IL-6R subunit beta; IL-6 receptor subunit beta; gp130, oncostatin M receptor; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; CDW130; CD130; CD antigen CD130; GP130; Interleukin-6 receptor subunit beta; interleukin 6 signal transducer; GP130/CD130 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR114H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for GP130/CD130 recombinant antibody
ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Residues 23-323 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human GP130/CD130 by western blot. Samples: Whole cell lysate (50 ug) from NCI-H226, MCF-7, IMR-90, Jurkat, HeLa, A-549, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 .)
WB (Western Blot)
(Detection of human GP130/CD130 by western blot. Samples: Whole cell lysate (50 ug) from NCI-H226, MCF-7, IMR-90, Jurkat, HeLa, A-549, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 .)
ICC (Immunocytochemistry)
(Detection of human GP130/CD130 in FFPE HeLa cells by immunocytochemistry. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human GP130/CD130 in FFPE HeLa cells by immunocytochemistry. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human GP130/CD130 in FFPE HUVEC-C cells by immunocytochemistry. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human GP130/CD130 in FFPE HUVEC-C cells by immunocytochemistry. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human GP130/CD130 in FFPE GaMG cells by immunocytochemistry. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human GP130/CD130 in FFPE GaMG cells by immunocytochemistry. Antibody: Rabbit anti-GP130/CD130 recombinant monoclonal antibody (AAA213585 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for GP130/CD130 recombinant antibody
GP130/CD130/IL6ST is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. [taken from NCBI Entrez Gene (Gene ID: 3572)].
Product Categories/Family for GP130/CD130 recombinant antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GP130/CD130 il6st (Catalog #AAA213585) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GP130/CD130 Recombinant Monoclonal Antibody [BLR114H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GP130/CD130 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GP130/CD130 il6st for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GP130/CD130, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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