WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with GPC3 antibody (RHE84602) at 1?ug/ml.Lane 1: Human GPC3 transfected HEK293 cell lysateLane 2: Non-transfected HEK293 cell lysateSecond Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 ug/mL.Predict MW: 63 kDa)
GPC3 recombinant antibody
Anti-Human GPC3 Antibody (YP7)
Protein A or G purified from cell culture supernatant.
Protein A or G purified from cell culture supernatant.
WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with GPC3 antibody (RHE84602) at 1?ug/ml.Lane 1: Human GPC3 transfected HEK293 cell lysateLane 2: Non-transfected HEK293 cell lysateSecond Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 ug/mL.Predict MW: 63 kDa)
WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with GPC3 antibody (RHE84602) at 1?ug/ml.Lane 1: Human GPC3 transfected HEK293 cell lysateLane 2: Non-transfected HEK293 cell lysateSecond Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 ug/mL.Predict MW: 63 kDa)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human GPC3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # RHE84602 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human GPC3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # RHE84602 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human GPC3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # RHE84602 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human GPC3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # RHE84602 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human GPC3 antibody.GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # RHE84602 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human GPC3 antibody.GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # RHE84602 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.)
Bioactivity
(Detects Human GPC3 in indirect ELISAs.)
Bioactivity
(Detects Human GPC3 in indirect ELISAs.)
NCBI and Uniprot Product Information
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Product Notes
The GPC3 gpc3 (Catalog #AAA120368) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human GPC3 Antibody (YP7) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPC3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the GPC3 gpc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPC3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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