IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GPx-4 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
GPx-4 recombinant antibody
Recombinant Anti-GPx-4 Rabbit mAb
Gene Names
GPX4; MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
GPx-4, Recombinant Antibody; Recombinant Anti-GPx-4 Rabbit mAb; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; PHGPx; Glutathione peroxidase 4; GPx-4; GSHPx-4; GPx-4 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of GPx-4 protein
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS, pH7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Applicable Applications for GPx-4 recombinant antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human GPx-4. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GPx-4 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GPx-4 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
WB (Western Blot)
(Western blot analysis of GPx-4 expression in HEK293T (A), MCF7 (B), mouse kidney (C), mouse testis (D), rat kidney (E) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD))
WB (Western Blot)
(Western blot analysis of GPx-4 expression in HEK293T (A), MCF7 (B), mouse kidney (C), mouse testis (D), rat kidney (E) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD))
Related Product Information for GPx-4 recombinant antibody
Recombinant rabbit monoclonal antibody to GPx-4
Product Categories/Family for GPx-4 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 22kDa
Calculated MW: 19kDa/22kDa
Calculated MW: 19kDa/22kDa
NCBI Official Full Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
NCBI Official Synonym Full Names
glutathione peroxidase 4
NCBI Official Symbol
GPX4
NCBI Official Synonym Symbols
MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
NCBI Protein Information
phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
UniProt Protein Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
UniProt Gene Name
GPX4
UniProt Synonym Gene Names
PHGPx; GPx-4; GSHPx-4
UniProt Entry Name
GPX4_HUMAN
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Product Notes
The GPx-4 gpx4 (Catalog #AAA266269) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-GPx-4 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPx-4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GPx-4 gpx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPx-4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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