IF (Immunofluorescence)
(Immunofluorescence staining of Human GRN in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human GRN monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Mouse anti-Human Granulin Monoclonal Antibody | anti-GRN antibody
Anti-Granulin Antibody, Mouse Monoclonal
Gene Names
GRN; GEP; GP88; PEPI; PGRN; CLN11; PCDGF
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, ELISA, ELISA
Purity
Protein A
Synonyms
Granulin, Antibody; Anti-Granulin Antibody, Mouse Monoclonal; Granulin/GRN/Progranulin Antibody, Mouse MAb; Granulin Precursor; Anti-CLN11 Antibody; Anti-GEP Antibody; Anti-GP88 Antibody; Anti-Granulin Antibody; Anti-PCDGF Antibody; Anti-PEPI Antibody; Anti-PGRN Antibody; Anti-Progranulin Antibody; anti-GRN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
2F3G11
Specificity
Human Granulin/GRN
Purity/Purification
Protein A
Form/Format
0.2um filtered solution in PBS
Applicable Applications for anti-GRN antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, ELISA
Immunogen
Recombinant Human Granulin protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granulin/GRN (rhGRN). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of Human GRN in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human GRN monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human GRN in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human GRN monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
References
Hoque M, et al. (2003) The growth factor granulin interacts with cyclin T1 and modulates P-TEFb-dependent transcription. Mol Cell Biol. 23(5): 1688-702.Bateman A, et al. (1990) Granulins, a novel class of peptide from leukocytes. Biochem Biophys Res Commun. 173(3): 1161-8.Trinh DP, et al. (1999) Epithelin/granulin growth factors: extracellular cofactors for HIV-1 and HIV-2 Tat proteins. Biochem Biophys Res Commun. 256(2): 299-306.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
granulins
NCBI Official Synonym Full Names
granulin
NCBI Official Symbol
GRN
NCBI Official Synonym Symbols
GEP; GP88; PEPI; PGRN; CLN11; PCDGF
NCBI Protein Information
granulins; PC cell-derived growth factor; acrogranin; granulin-epithelin; proepithelin; progranulin
UniProt Protein Name
Granulins
UniProt Gene Name
GRN
UniProt Synonym Gene Names
PEPI
UniProt Entry Name
GRN_HUMAN
Similar Products
Product Notes
The GRN grn (Catalog #AAA255206) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Granulin Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Granulin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, ELISA. Researchers should empirically determine the suitability of the GRN grn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Granulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.