Mouse GRANZYME B Monoclonal Antibody | anti-GZMB antibody

MOUSE ANTI GRANZYME B

Cat. Num. AAA49332

• Gene Names: GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: GRANZYME B, Antibody; MOUSE ANTI GRANZYME B; anti-GZMB antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: GB11
• Form/Format: Purified IgG - liquid ; Tris buffered saline
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 247

• Applicable Applications for anti-GZMB antibody: FCM/FACS (Flow Cytometry), ELISA
• Perservative Stabilisers: 0.09% Sodium azide (NaN₃)
• RRID: AB_2114582
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Immunogen: Purified human Granzyme B.
• Buffer Solution: Tris buffered saline
• Target Species: Human
• Reactivity Note: Reacts with: Chimpanzee, Monkey, Rhesus Monkey; N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Application Data

(Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B as a capture reagent and biotinylated Mouse anti Human granzyme B as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Human plasma samples are diluted i:2 (green) and 1:4 (blue))

Related Product Information for anti-GZMB antibody

Mouse anti Granzyme B antibody, clone GB11 recognizes the serine protease Granzyme B, important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).
Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death.
Mouse anti Granzyme B antibody, clone GB11 is suitable for the detection of Granzyme B expressing cells by flow cytometry. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B.

NCBI and Uniprot Product Information

• NCBI GI #: 221625528
• NCBI GeneID: 3002
• NCBI Accession #: NP_004122.2
• NCBI GenBank Nucleotide #: NM_004131.4
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• NCBI Official Full Name: granzyme B
• NCBI Official Synonym Full Names: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B; C11; CTLA-1; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic serine protease B; fragmentin 2; fragmentin-2; human lymphocyte protein
• UniProt Protein Name: Granzyme B
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
• UniProt Entry Name: GRAB_HUMAN