WB (Western Blot)
(Western blot analysis of lysates from PC-3 cells using GSDMD (Full length+C terminal) Rabbit mAb (AAA282854) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
Rabbit anti-Human GSDMD Monoclonal Antibody | anti-GSDMD antibody
GSDMD (Full length+C terminal) Rabbit mAb
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
GSDMD, Antibody; GSDMD (Full length+C terminal) Rabbit mAb; DF5L; DFNA5L; FKSG10; GSDMDC1; GSDMD; anti-GSDMD antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
DGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
Applicable Applications for anti-GSDMD antibody
ELISA, WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human GSDMD (NP_079012.3).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from PC-3 cells using GSDMD (Full length+C terminal) Rabbit mAb (AAA282854) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of lysates from PC-3 cells using GSDMD (Full length+C terminal) Rabbit mAb (AAA282854) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit mAb (AAA282854) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37? for overnight and LPS (1 ug/ml) at 37? for 6 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit mAb (AAA282854) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37? for overnight and LPS (1 ug/ml) at 37? for 6 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of lysates from A-431 cells, using GSDMD Rabbit mAb (AAA282854) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of lysates from A-431 cells, using GSDMD Rabbit mAb (AAA282854) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)
Related Product Information for anti-GSDMD antibody
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
Product Categories/Family for anti-GSDMD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 53kDa
Observed MW: 53kDa/22kDa/
Observed MW: 53kDa/22kDa/
UniProt Protein Name
Gasdermin-D
UniProt Gene Name
GSDMD
UniProt Synonym Gene Names
DFNA5L; GSDMDC1
UniProt Entry Name
GSDMD_HUMAN
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Product Notes
The GSDMD gsdmd (Catalog #AAA282854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GSDMD (Full length+C terminal) Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSDMD can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GSDMD gsdmd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DGPAGAVLEC LVLSSGMLVP ELAIPVVYLL GALTMLSETQ HKLLAEALES QTLLGPLELV GSLLEQSAPW QERSTMSLPP GLLGNSWGEG APAWVLLDEC GLELGEDTPH VCWEPQAQGR MCALYASLAL LSGLSQEPH. It is sometimes possible for the material contained within the vial of "GSDMD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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