WB (Western Blot)
(Detection of mouse GSK3-beta by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GSK3-beta recombinant monoclonal antibody (AAA213647 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
GSK3-beta recombinant antibody
Rabbit anti-GSK3-beta Recombinant Monoclonal Antibody [BLR223K]
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
GSK3-beta, Recombinant Antibody; Rabbit anti-GSK3-beta Recombinant Monoclonal Antibody [BLR223K]; serine/threonine-protein kinase GSK3B; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; Glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta; GSK3-beta recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR223K
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
400ug/ml (varies by lot)
Applicable Applications for GSK3-beta recombinant antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 370 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse GSK3-beta by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GSK3-beta recombinant monoclonal antibody (AAA213647 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse GSK3-beta by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GSK3-beta recombinant monoclonal antibody (AAA213647 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human GSK3-beta by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, MOLT-4, HeLa, HEK293T, and MDA-MB-435 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GSK3-beta recombinant monoclonal antibody (AAA213647 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human GSK3-beta by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, MOLT-4, HeLa, HEK293T, and MDA-MB-435 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GSK3-beta recombinant monoclonal antibody (AAA213647 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human GSK3-beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GSK3-beta recombinant monoclonal antibody (AAA213647 lot 1) used for IP at 15 uL/mg lysate. GSK3-beta was also immunoprecipitated by a second antibody against a different epitope of GSK3-beta . For blotting immunoprecipitated GSK3-beta, AAA213647 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human GSK3-beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GSK3-beta recombinant monoclonal antibody (AAA213647 lot 1) used for IP at 15 uL/mg lysate. GSK3-beta was also immunoprecipitated by a second antibody against a different epitope of GSK3-beta . For blotting immunoprecipitated GSK3-beta, AAA213647 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for GSK3-beta recombinant antibody
Glycogen synthase kinase 3 beta (GSK3beta) is a proline-directed serine-threonine kinase identified as a kinase responsible for the phosphorylation and inactivation of glycogen synthase. GSK3beta is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. In addition to the Wnt-signaling pathway, GSK3beta is also involved in the Hedgehog, Notch and insulin signaling pathways.
Product Categories/Family for GSK3-beta recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,034 Da
NCBI Official Full Name
glycogen synthase kinase-3 beta isoform 1
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
GSK3B
NCBI Protein Information
glycogen synthase kinase-3 beta
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
GSK3B
UniProt Synonym Gene Names
GSK-3 beta
UniProt Entry Name
GSK3B_HUMAN
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Product Notes
The GSK3-beta gsk3b (Catalog #AAA213647) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GSK3-beta Recombinant Monoclonal Antibody [BLR223K] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3-beta can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GSK3-beta gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3-beta, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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