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IF (Immunofluorescence)
(AAA328874 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328874 1:200) and Rabbit anti-beta tubulin Ab(AF7011 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-Rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.)
Mouse GSK3A/B Monoclonal Antibody | anti-GSK3A antibody
GSK3A/B Antibody [AFfirm036]
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
GSK3A/B, Antibody; GSK3A/B Antibody [AFfirm036]; Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3B_HUMAN; GSK3beta isoform; Serine/threonine-protein kinase GSK3B; anti-GSK3A antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AFfirm036
Specificity
GSK3B antibody detects endogenous levels of total GSK3B.
Purity/Purification
Peptide affinity purification
Form/Format
IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
~1mg/ml (varies by lot)
Sequence Length
420
Applicable Applications for anti-GSK3A antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human GSK3 beta.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328874 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328874 1:200) and Rabbit anti-beta tubulin Ab(AF7011 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-Rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(AAA328874 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328874 1:200) and Rabbit anti-beta tubulin Ab(AF7011 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-Rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA328874 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Mouse Ab was used as the secondary Ab.)
IHC (Immunohistochemistry)
(AAA328874 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Mouse Ab was used as the secondary Ab.)
IHC (Immunohistochemisry)
(AAA328874 at 1/100 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA328874 at 1/100 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328874 at 1/100 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328874 at 1/100 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis on various lysates using GSK3B mouse monoclonal antibody.lane1 mouse testis with blocking peptide,lane2 mouse testis,lane3 rat testislane4 Hela cells)
WB (Western Blot)
(Western blot analysis on various lysates using GSK3B mouse monoclonal antibody.lane1 mouse testis with blocking peptide,lane2 mouse testis,lane3 rat testislane4 Hela cells)
Related Product Information for anti-GSK3A antibody
Glycogen synthase kinase 3 (GSK-3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK-3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK-3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK-3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK-3 are ubiquitously expressed, although particularly high levels of GSK-3beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46 kDa
NCBI Official Full Name
glycogen synthase kinase-3 beta isoform 2
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
GSK3B
NCBI Protein Information
glycogen synthase kinase-3 beta
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
GSK3B
UniProt Synonym Gene Names
GSK-3 beta
UniProt Entry Name
GSK3B_HUMAN
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Product Notes
The GSK3A gsk3b (Catalog #AAA328874) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GSK3A/B Antibody [AFfirm036] reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3A/B can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GSK3A gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3A/B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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