ELISA
(H5N1 HA 1 Antibody (1 ug/ml)specifically recognizes H5N1 /HA1 recombinant protein, but not interacted H1N1 /HA1 and H3N2/HA1 recombinant protein in ELISA.)
Mouse anti-Human H5N1/HA1 Monoclonal Antibody | anti-H5N1/HA1 antibody
H5N1/HA1 antibody
Reactivity
Human
Applications
Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
H5N1/HA1, Antibody; H5N1/HA1 antibody; Hemagglutinin; Influenza A virus (A/Vietnam/HN31242/2007H5N1) haemagglutinin; anti-H5N1/HA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 heavy chain and k light chain
Clone Number
AT2B7
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
567
Applicable Applications for anti-H5N1/HA1 antibody
WB (Western Blot), ELISA
Antigen Species
Influenza A-H5N1
Immunogen
Recombinant human H5N1/HA1 (17-338aa) purified from Baculovirus
Clone Note
Anti-human H5N1/HA1 mAb, clone 2B7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human H5N1/HA1 protein.
Usage
The antibody has been tested by ELISA,Western Blot analysis to assure specifity and reactivity. Since application varies, however each investigation should be titrated by the reagent to obtain optimal results.
Preparation and Storage
Can be stored at 4°C.
For long term storage, aliquot and store at -20°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C.
Avoid repeated freezing and thawing cycles.
ELISA
(H5N1 HA 1 Antibody (1 ug/ml)specifically recognizes H5N1 /HA1 recombinant protein, but not interacted H1N1 /HA1 and H3N2/HA1 recombinant protein in ELISA.)
ELISA
(H5N1 HA 1 Antibody (1 ug/ml)specifically recognizes H5N1 /HA1 recombinant protein, but not interacted H1N1 /HA1 and H3N2/HA1 recombinant protein in ELISA.)
WB (Western Blot)
(H5N1/HA1 recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human H5N1/HA1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(H5N1/HA1 recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human H5N1/HA1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-H5N1/HA1 antibody
H5N1 is a subtype of the species Influenza A virus of the Influenzavirus A genus of the Orthomyxoviridae family. consists of single-stranded eight-segment negative-sense genomic RNAs, helical viral ribonucleoprotein (RNP) complexes (RNA segments NP, PB2, PB1 and PA), three viral envelope proteins (hemagglutinin [HA], neuraminidase [NA], and M2 ion channel), and a maxtir (M1) protein. Influenza A viruses are further classified into 16 HA (H1-H16) and 9 NA (N1-N9) serotypes based on the antigenic characteristics of HA and NA envelope glycoproteins. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.
References
Song D., et al. (2008) Emerg. Infect. Dis. 14:741-746; Li S., et al. (2010) Infect. Genet. Evol. 10:1286-1288; Horimoto T, Kawaoka Y (2005) Influenza: lessons from past pandemics, warnings from current incidents. Nat Rev Microbiol 3: 591-600.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
hemagglutinin
UniProt Protein Name
Hemagglutinin
UniProt Gene Name
H
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Product Notes
The H5N1/HA1 h (Catalog #AAA48096) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The H5N1/HA1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's H5N1/HA1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the H5N1/HA1 h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H5N1/HA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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