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product-image-AAA210714_WB10.jpg WB (Western Blot) (Detection of HA-tagged protein by western blot of lysate from HEK293 transfected with Met-HA-IDO1, HEK293 transfected with IDO1-HA-V5 (Internal Tag), and HEK293 transfected with IDO1-V5-HA (C-terminal Tag) cells. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-IDO1 recombinant monoclonal antibody .)

HA Tag Recombinant Antibody | HA recombinant antibody

Rabbit anti-HA Tag Recombinant Monoclonal Antibody [BLRE02G]

Applications
ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
HA Tag, Recombinant Antibody; Rabbit anti-HA Tag Recombinant Monoclonal Antibody [BLRE02G]; HA recombinant antibody
Ordering
Host
Rabbit
Clonality
Monoclonal
Clone Number
BLRE02G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for HA recombinant antibody
ELISA, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
HA Epitope tag (YPYDVPDYA)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of HA-tagged protein by western blot of lysate from HEK293 transfected with Met-HA-IDO1, HEK293 transfected with IDO1-HA-V5 (Internal Tag), and HEK293 transfected with IDO1-V5-HA (C-terminal Tag) cells. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-IDO1 recombinant monoclonal antibody .)

product-image-AAA210714_WB10.jpg WB (Western Blot) (Detection of HA-tagged protein by western blot of lysate from HEK293 transfected with Met-HA-IDO1, HEK293 transfected with IDO1-HA-V5 (Internal Tag), and HEK293 transfected with IDO1-V5-HA (C-terminal Tag) cells. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-IDO1 recombinant monoclonal antibody .)

IP (Immunoprecipitation)

(Detection of HA Tag by western blot of immunoprecipitates from HEK293 transfected with Met-HA-IDO1 lysate. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1) and Rabbit anti-IDO1 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blotting immunoprecipitated HA Tag, AAA210714 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

product-image-AAA210714_IP11.jpg IP (Immunoprecipitation) (Detection of HA Tag by western blot of immunoprecipitates from HEK293 transfected with Met-HA-IDO1 lysate. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1) and Rabbit anti-IDO1 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blotting immunoprecipitated HA Tag, AAA210714 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

ICC (Immunocytochemistry)

(Detection of HA Tag in FFPE HEK293 cells expressing HA tagged nuclear protein by ICC. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

product-image-AAA210714_ICC13.jpg ICC (Immunocytochemistry) (Detection of HA Tag in FFPE HEK293 cells expressing HA tagged nuclear protein by ICC. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of HA Tag in FFPE HEK293 cells expressing HA tagged cytoplasmic protein by ICC. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

product-image-AAA210714_ICC15.jpg ICC (Immunocytochemistry) (Detection of HA Tag in FFPE HEK293 cells expressing HA tagged cytoplasmic protein by ICC. Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody (AAA210714 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for HA recombinant antibody
The HA (human influenza hemagglutinin) epitope tag is frequently engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized HA epitope represents the amino acid sequence YPYDVPDYA.
Product Categories/Family for HA recombinant antibody

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Product Notes

The HA (Catalog #AAA210714) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HA Tag can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA Tag, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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