Mouse anti-Human Haptoglobin Monoclonal Antibody | anti-HP antibody

Mouse anti Human Haptoglobin

Cat. Num. AAA50426

• Gene Names: HP; BP; HPA1S; HP2ALPHA2
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: Haptoglobin, Antibody; Mouse anti Human Haptoglobin; Haptoglobin antibody | E07-10E9; HAPTOGLOBIN; anti-HP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: E07-10E9
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN₃); Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)

• Applicable Applications for anti-HP antibody: ELISA
• Immunogen: Native haptoglobin purified from human plasma
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Preparation and Storage: It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA

(Sandwich ELISA analysis of human haptoglobin using Mouse anti Human haptoglobin (AAA50426) as a capture reagent and biotinylated Mouse anti Human haptoglobin as a detection reagent with purified native human haptoglobin as the antigen. Detecti)

Related Product Information for anti-HP antibody

Mouse anti Human haptoglobin antibody, clone E07-10E9, recognizes haptoglobin, an alpha sialoglycoprotein. Synthesis of haptoglobin is induced by IL-6, IL-1 beta and TNF and it is produced as a pro-protein before it is cleaved in the endoplasmic reticulum by C1r-like protein, to generate the mature form (Redmond et al. 2018). Haptoglobin is composed of two peptide chains referred to as the alpha and beta chains. It is largely expressed by hepatocytes, but is also expressed to a lesser extent in lung, skin and kidney tissues (Vanuytsel et al. 2013). A key role of haptoglobin is its hemoglobin binding capacity, which is utilized during hemolysis to prevent iron loss and subsequent kidney damage. The haptoglobin-hemoglobin complex is removed by the CD163 receptor expressed on the surface of monocytes and macrophages (Sadrzadeh & Bozorgmehr 2004). Haptoglobin also plays roles in the innate and adaptive immune system and has anti-inflammatory activity such as inhibiting monocyte production of TNF, IL-11 and IL-12 (Arredouani et al. 2005).

The purified Mouse anti Human haptoglobin antibody, clone E07-10E9 can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human haptoglobin antibody, clone C08-1E10 as the detection antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 386783
• NCBI GeneID: 3240
• UniProt Accession #: P00738; P00737; Q0VAC4; Q2PP15; Q3B7J0; Q6LBY9; Q9UC67; B0AZL5
• NCBI Official Full Name: haptoglobin
• NCBI Official Synonym Full Names: haptoglobin
• NCBI Official Symbol: HP
• NCBI Official Synonym Symbols: BP; HPA1S; HP2ALPHA2
• NCBI Protein Information: haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide
• UniProt Protein Name: Haptoglobin
• UniProt Gene Name: HP
• UniProt Entry Name: HPT_HUMAN