FCM/FACS (Flow Cytometry)
(Fig-2: Cell surface flow analysis of hCD98 on human PBMCs (Monocytes gated) using 1 ug/ 10^6 cells. Green represents isotype control; red represents anti-hCD98 antibody (10-4168). Goat anti-mouse PE conjugated secondary antibody was used.. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
Mouse anti-Human hCD98 Monoclonal Antibody | anti-hCD98 antibody
Monoclonal Antibody to hCD98 (Clone: ABM5A27)
Gene Names
SLC3A2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
PurifiedProtein G Chromatography
Synonyms
hCD98, Antibody; Monoclonal Antibody to hCD98 (Clone: ABM5A27); 4F2 cell-surface antigen heavy chain; 4F2 heavy chain antigen; Lymphocyte activation antigen 4F2 large subunit; Solute carrier family 3 member 2; anti-hCD98 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, Kappa
Clone Number
ABM5A27
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
631
Applicable Applications for anti-hCD98 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Antigen
hCD98
Immunogen Information
A partial length recombinant protein (a.a 162-371) of hCD98 was used as the immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.
FCM/FACS (Flow Cytometry)
(Fig-2: Cell surface flow analysis of hCD98 on human PBMCs (Monocytes gated) using 1 ug/ 10^6 cells. Green represents isotype control; red represents anti-hCD98 antibody (10-4168). Goat anti-mouse PE conjugated secondary antibody was used.. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Fig-2: Cell surface flow analysis of hCD98 on human PBMCs (Monocytes gated) using 1 ug/ 10^6 cells. Green represents isotype control; red represents anti-hCD98 antibody (10-4168). Goat anti-mouse PE conjugated secondary antibody was used.. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
WB (Western Blot)
(Fig-1: Western blot analysis of hCD98. Anti-hCD98 antibody (Clone: ABM5A27) was used at 4 ug/ml on human Kidney lysate.)
WB (Western Blot)
(Fig-1: Western blot analysis of hCD98. Anti-hCD98 antibody (Clone: ABM5A27) was used at 4 ug/ml on human Kidney lysate.)
Related Product Information for anti-hCD98 antibody
CD98 was originally identified as a cell surface antigen associated with lymphocyte activation defined by 4F2 mAb; it is expressed in proliferating normal tissues and in almost all tumor cells. CD98 heterodimer consists of a type II single-pass transmembrane heavy chain (CD98hc, also known as 4F2 antigen heavy chain or FRP-1; encoded by the genes SLC3A2 and Slc3a2 for human and mouse, respectively) of ~80-85 kDa that is disulfide-linked with a multi-pass light chain of ~40 kDa. CD98 hc (heavy chain) is a type II transmembrane glycoprotein that is disulfide-linked to a non-glycosylated light chain of a member of the permease family. It has been reported that CD98 was functionally involved in lymphocyte activation, cell proliferation, and malignant transformation. CD98 forms the large neutral amino acid transporter that is a heterodimeric membrane transport protein to transport branched-chain (valine, leucine, isoleucine) and aromatic (tryptophan, tyrosine) amino acids. Intestinal epithelial cells express CD98 under the physiological conditions. Recent reports indicate that CD98 is involved in the intestinal inflammation, which can be upregulated by interferon (IFN)-gamma in the intestine.
Product Categories/Family for anti-hCD98 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,123 Da
NCBI Official Full Name
4F2 cell-surface antigen heavy chain isoform b
NCBI Official Synonym Full Names
solute carrier family 3 member 2
NCBI Official Symbol
SLC3A2
NCBI Official Synonym Symbols
4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
NCBI Protein Information
4F2 cell-surface antigen heavy chain
UniProt Protein Name
4F2 cell-surface antigen heavy chain
UniProt Gene Name
SLC3A2
UniProt Synonym Gene Names
MDU1; 4F2hc
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Product Notes
The hCD98 slc3a2 (Catalog #AAA78423) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to hCD98 (Clone: ABM5A27) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's hCD98 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the hCD98 slc3a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hCD98, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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