Mouse HCN1 Monoclonal Antibody | anti-Hcn1 antibody

HCN1 Antibody: ATTO 390

Cat. Num. AAA102885

• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Protein G purified
• Synonyms: HCN1, Antibody; HCN1 Antibody: ATTO 390; BCNG-1; BCNG1; Brain cyclic nucleotide gated channel 1; Brain cyclic nucleotide-gated channel 1; HAC2; HCN1; HCN1_HUMAN; Hyperpolarization activated cyclic nucleotide gated potassium channel 1; anti-Hcn1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S70
• Specificity: Detects ~100kDa. No cross reactivity with HCN2.
• Purity/Purification: Protein G purified
• Form/Format: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 910

• Applicable Applications for anti-Hcn1 antibody: ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Conjugate: ATTO 390
• Immunogen: Fusion protein amino acids 778-910 (C-terminus) of rat HCN1.
• Cellular Localization: Membrane
• Research Area(s): Neuroscience , Ion Channels, Cyclic Necleotide-Gated Ion Channels, Cardiovascular System, Heart
• Function: Hyperpolarization-activated cation channels of the HCN gene family , such as HCN1, play a crucial role in the regulations of cell excitability. Importantly , they contribute to spontaneous rhythmic activity in both the heart and brain.
• Preparation and Storage: -20 degree C

IHC (Immunohistochemistry)

(Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons.)

IHC (Immunohistochemisry)

(Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

WB (Western Blot)

(Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.)

Product Categories/Family for anti-Hcn1 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232.

NCBI and Uniprot Product Information

• NCBI GI #: 16758108
• NCBI GeneID: 84390
• NCBI Accession #: NP_445827.1
• NCBI GenBank Nucleotide #: NM_053375.1
• UniProt Accession #: Q9JKBO; Q9JKB0
• NCBI Official Full Name: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
• NCBI Official Synonym Full Names: hyperpolarization-activated cyclic nucleotide-gated potassium channel 1
• NCBI Official Symbol: Hcn1
• NCBI Protein Information: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
• UniProt Protein Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
• UniProt Gene Name: Hcn1
• UniProt Entry Name: HCN1_RAT