WB (Western Blot)
(HDAC2 mAb (Clone 3F3) tested by Western blot. Detection of HDAC2 by Western blot. The analysis was performed using HeLa nuclear extract and HDAC2 mAb (Clone 3F3) at a 1:5,000 dilution.)
Mouse HDAC2 Monoclonal Antibody | anti-HDAC2 antibody
HDAC2 antibody (mAb)(Clone 3F3)
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Culture Supernatant
Synonyms
HDAC2, Antibody; HDAC2 antibody (mAb)(Clone 3F3); HD2; RPD3; YAF1; anti-HDAC2 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F3
Purity/Purification
Culture Supernatant
Form/Format
Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version of this antibody that was purified by Protein A Chromatography is also available.
Applicable Applications for anti-HDAC2 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation)
Immunogen
This HDAC2 antibody was raised against a KLH-conjugated peptide corresponding to amino acids 473-488 of human HDAC2.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
WB (Western Blot)
(HDAC2 mAb (Clone 3F3) tested by Western blot. Detection of HDAC2 by Western blot. The analysis was performed using HeLa nuclear extract and HDAC2 mAb (Clone 3F3) at a 1:5,000 dilution.)
WB (Western Blot)
(HDAC2 mAb (Clone 3F3) tested by Western blot. Detection of HDAC2 by Western blot. The analysis was performed using HeLa nuclear extract and HDAC2 mAb (Clone 3F3) at a 1:5,000 dilution.)
IF (Immunofluorescence)
(HDAC2 mAb (Clone 3F3) tested by immunofluorescence. Detection of HDAC2 by immunofluorescence. HeLa cells were stained with HDAC2 mAb (Clone 3F3) at a 1:1,000 dilution. Red: HDAC2 mAb (Clone 3F3) Green: alpha-Tubulin mouse monoclonal (Clone 5-B-1-2) conjugated to Chromeo 488.)
IF (Immunofluorescence)
(HDAC2 mAb (Clone 3F3) tested by immunofluorescence. Detection of HDAC2 by immunofluorescence. HeLa cells were stained with HDAC2 mAb (Clone 3F3) at a 1:1,000 dilution. Red: HDAC2 mAb (Clone 3F3) Green: alpha-Tubulin mouse monoclonal (Clone 5-B-1-2) conjugated to Chromeo 488.)
Related Product Information for anti-HDAC2 antibody
Short Description: Our HDAC2 antibody (mAb)(Clone 3F3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human, Mouse and Rat samples.
Background: HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.
Background: HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.
Product Categories/Family for anti-HDAC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 54 kDa
Observed: 52 kDa
Observed: 52 kDa
NCBI Official Full Name
histone deacetylase 2
NCBI Official Synonym Full Names
histone deacetylase 2
NCBI Official Symbol
HDAC2
NCBI Official Synonym Symbols
HD2; RPD3; YAF1
NCBI Protein Information
histone deacetylase 2; YY1-associated factor 1; transcriptional regulator homolog RPD3
UniProt Protein Name
Histone deacetylase 2
UniProt Gene Name
HDAC2
UniProt Synonym Gene Names
HD2
UniProt Entry Name
HDAC2_HUMAN
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Product Notes
The HDAC2 hdac2 (Catalog #AAA59875) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HDAC2 antibody (mAb)(Clone 3F3) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the HDAC2 hdac2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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