Mouse anti-Human Hemoglobin Monoclonal Antibody | anti-LOC541815 antibody

Mouse Monoclonal [clone M1709Hg2] (IgG1) to Human Hemoglobin

Cat. Num. AAA52542

• Reactivity: Human
• Applications: Lateral Flow, ELISA, Immunohistochemistry
• Purity: Ion exchange chromatography
• Synonyms: Hemoglobin, Antibody; Mouse Monoclonal [clone M1709Hg2] (IgG1) to Human Hemoglobin; Anti-Hemoglobin Antibody (clone M1709Hg2) IHC-plus; Hemoglobin; Human Hemoglobin; anti-LOC541815 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M1709Hg2
• Specificity: Human Hemoglobin
• Purity/Purification: Ion exchange chromatography
• Form/Format: 50 mM NaCl, 10mM PBS (pH 8.0) with 0.1% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 165

• Applicable Applications for anti-LOC541815 antibody: LF (Lateral Flow), ELISA, IHC (Immunohistochemistry)
• Immunogen Species: Hemoglobin antibody was raised against Human
• Immunogen Description: Human hemoglobin.
• Target: hemoglobin
• Antigen Type: Purified protein
• Immunogen: Hemoglobin antibody was raised against human hemoglobin.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

IHC (Immunohistochemistry)

(Anti-Hemoglobin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 162462053
• NCBI GeneID: 541815
• NCBI Accession #: NP_001104966.1
• NCBI GenBank Nucleotide #: NM_001111496.1
• NCBI Official Full Name: hemoglobin
• NCBI Official Symbol: LOC541815
• NCBI Protein Information: hemoglobin; GRMZM2G067402; non-symbiotic hemoglobin; reversible oxygen binding