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WB (Western Blot)
(Detection of human Hepassocin/FGL1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, SHP-77, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Hepassocin/FGL1 recombinant monoclonal antibody (AAA213642 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
Hepassocin/FGL1 Recombinant Antibody | FGL1 recombinant antibody
Rabbit anti-Hepassocin/FGL1 Recombinant Monoclonal Antibody [BLR210K]
Gene Names
FGL1; HFREP1; HP-041; LFIRE1; LFIRE-1
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Hepassocin/FGL1, Recombinant Antibody; Rabbit anti-Hepassocin/FGL1 Recombinant Monoclonal Antibody [BLR210K]; LFIRE1; LFIRE-1; HP-041; HFREP-1; HFREP1; hepatocyte-derived fibrinogen-related protein 1; hepatocellular carcinoma-related sequence; hepassocin; fibrinogen-like protein 1; liver fibrinogen-related protein 1; Fibrinogen-like protein 1; fibrinogen like 1; FGL1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR210K
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for FGL1 recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 18-312 (full length)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Hepassocin/FGL1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, SHP-77, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Hepassocin/FGL1 recombinant monoclonal antibody (AAA213642 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Hepassocin/FGL1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, SHP-77, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Hepassocin/FGL1 recombinant monoclonal antibody (AAA213642 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human Hepassocin/FGL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Hepassocin/FGL1 recombinant monoclonal antibody (AAA213642 lot 1) used for IP at 12 ul/mg lysate. Hepassocin/FGL1 was also immunoprecipitated by a second antibody against a different epitope of Hepassocin/FGL1. For blotting immunoprecipitated Hepassocin/FGL1, AAA213642 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human Hepassocin/FGL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Hepassocin/FGL1 recombinant monoclonal antibody (AAA213642 lot 1) used for IP at 12 ul/mg lysate. Hepassocin/FGL1 was also immunoprecipitated by a second antibody against a different epitope of Hepassocin/FGL1. For blotting immunoprecipitated Hepassocin/FGL1, AAA213642 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohiostchemistry)
(Detection of human Hepassocin/FGL1 by immunohistochemistry. Sample: FFPE section of liver carcinoma. Antibody: Rabbit anti-FGL-1 recombinant monoclonal antibody (AAA213642 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohiostchemistry)
(Detection of human Hepassocin/FGL1 by immunohistochemistry. Sample: FFPE section of liver carcinoma. Antibody: Rabbit anti-FGL-1 recombinant monoclonal antibody (AAA213642 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human Hepassocin/FGL1 by immunocytochemistry. Sample: FFPE section of SHP-77 cells. Antibody: Rabbit anti-FGL-1 recombinant monoclonal antibody (AAA213642 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human Hepassocin/FGL1 by immunocytochemistry. Sample: FFPE section of SHP-77 cells. Antibody: Rabbit anti-FGL-1 recombinant monoclonal antibody (AAA213642 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Related Product Information for FGL1 recombinant antibody
Hepassocin, also known as Fibrinogen-like 1 (FGL1), is a member of the fibrinogen family whose members share a fibrinogen domain at their C-termini. However, it lacks the platelet-binding site, cross-linking region and thrombin-sensitive site which are necessary for fibrin clot formation. Hepassocin/FGL1 is secreted by hepatocytes and may play a role in the development of hepatocellular carcinomas. Hepassocin/FGL1 is an immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 [taken from NCBI Entrez Gene (Gene ID: 2267)].
Product Categories/Family for FGL1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,379 Da
NCBI Official Full Name
fibrinogen-like protein 1
NCBI Official Synonym Full Names
fibrinogen like 1
NCBI Official Symbol
FGL1
NCBI Official Synonym Symbols
HFREP1; HP-041; LFIRE1; LFIRE-1
NCBI Protein Information
fibrinogen-like protein 1
UniProt Protein Name
Fibrinogen-like protein 1
UniProt Gene Name
FGL1
UniProt Synonym Gene Names
HFREP1; HFREP-1; LFIRE-1
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Product Notes
The FGL1 fgl1 (Catalog #AAA213642) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Hepassocin/FGL1 Recombinant Monoclonal Antibody [BLR210K] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hepassocin/FGL1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FGL1 fgl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepassocin/FGL1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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