Mouse Hepatocyte Specific Antigen/ Hep-Par1 Monoclonal Antibody

Hepatocyte Specific Antigen/ Hep-Par1

Cat. Num. AAA13560

• Reactivity: Human, Dog, Cat
• Synonyms: Hepatocyte Specific Antigen/ Hep-Par1, Antibody; Hepatocyte Specific Antigen/ Hep-Par1; anti-Hepatocyte Specific Antigen/ Hep-Par1 antibody

• Host: Mouse
• Reactivity: Human, Dog, Cat
• Clonality: Monoclonal
• Isotype: IgG1/K
• Clone Number: OCH1E5
• Form/Format: Anti-Hepatocyte Specific Antigen is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Application Notes: Specimen Preparation:; Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using a ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.; Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Immunogen: Formalin-fixed, failed human allograft liver that was mechanically disrupted.
• Precautions: 1. This product contains <0.1% sodium azide (NaN₃) as a preservative. Ensure proper handling procedures are used with this reagent.; 2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 3. Dispose of unused solution with copious amount of water.; 4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 7. For additional safety information refer to Safety Data Sheet for this product.; 8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL”.
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides.; 2. Air dry for 2 hours at 58°C.; 3. Deparaffinize, dehydrate and rehydrate tissues.; 4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.; 5. Any of three heating methods may be used:; a. TintoRetriever Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99°C. Incubate for 30-60 minutes.; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.; 8. Wash slides with ImmunoDNA washer or DI water.; 9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution.
• Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls.
• Reactivity Note: Paraffin, Frozen
• Control: Liver, Liver, Carcinoma
• Localization: Cytoplasmic
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter.
• Stability: This product is stable up to the expiration date on the product label. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
• Preparation and Storage: Store at 2 to 8°C. (Control slides: Store at 20-25°C).

Recommended IHC Protocol

IHC (Immunohistochemistry)

(IHC of Hepatocyte Specific Antigen on an FFPE Liver Tissue)

Dilution Information

Related Product Information for anti-Hepatocyte Specific Antigen/ Hep-Par1 antibody

This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.
Hepatocyte Specific Antigen (HSA or Hep Par 1) has been demonstrated consistently in the vast majority of Hepatocellular Carcinomas. Studies have shown the utility of HSA in the differential identification of Hepatocellular Carcinoma, Cholangiocarcinoma and Hepatoblastomas.

HSA recognizes both benign and malignant liver derived tissues including such tumors as Hepatoblastoma, Hepatocellular Carcinoma, and Hepatic Adenoma. It recognizes both normal adult and fetal liver tissue. The typical pattern is a granular cytoplasmic staining. This antibody is useful in differentiating Hepatocellular Carcinomas with adenoid features from Adenocarcinomas, either primary in the liver or metastatic lesions to the liver. In recognizing Hepatoblastoma, it is useful in differentiating this entity from other small round cell tumors.