Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using HER-2 Mouse Monoclonal Antibody (ERBB2/2453). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse HER-2/c-erbB-2/neu/CD340 Monoclonal Antibody | anti-HER-2 antibody
HER-2/c-erbB-2/neu/CD340
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Synonyms
HER-2/c-erbB-2/neu/CD340, Antibody; HER-2/c-erbB-2/neu/CD340; p185; CD340; Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog; anti-HER-2 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
ERBB2/2453
Specificity
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-HER-2 antibody
IHC (Immunohistochemistry)
Immunogen
Recombinant human HER-2 protein fragment (around aa 311-462)
Cellular Localization
Cell Surface
Hu-chromosome Location
17q12
Positive Control
SKBR-3 Cells or Breast Cancers.
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using HER-2 Mouse Monoclonal Antibody (ERBB2/2453). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using HER-2 Mouse Monoclonal Antibody (ERBB2/2453). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis HER-2 Mouse Monoclonal Antibody (ERBB2/2453).Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis HER-2 Mouse Monoclonal Antibody (ERBB2/2453).Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Human Breast Carcinoma stained with HER-2 Mouse Monoclonal Antibody (ERBB2/2453).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Human Breast Carcinoma stained with HER-2 Mouse Monoclonal Antibody (ERBB2/2453).)
References
1. Utrilla JC, et al. Histopathology. 34: 60-65 (1999). Wright C, et al. British Journal of Cancer. 65: 118-121 (1992). Corbett IP, et al. Journal of Pathology. 161: 15-25 (1990).
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Product Notes
The HER-2 (Catalog #AAA214644) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HER-2/c-erbB-2/neu/CD340 reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's HER-2/c-erbB-2/neu/CD340 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the HER-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HER-2/c-erbB-2/neu/CD340, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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