WB (Western Blot)
(Western Blot using anti-HER2/neu antibody SER4 at 2ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Mouse anti-Human HER2/neu Monoclonal Antibody | anti-HER2/neu antibody
Anti-HER2/neu [SER4], Mouse IgG1, Kappa
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human
Applications
ELISA
Purity
Protein A affinity purified
Synonyms
HER2/neu, Antibody; Anti-HER2/neu [SER4], Mouse IgG1, Kappa; ERBB2; Receptor tyrosine-protein kinase erbB-2; human epidermal growth factor receptor 2; anti-HER2/neu antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Clone Number
SER4
Specificity
The antibody binds specifically to the extracellular domain of human ErbB-2.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HER2/neu antibody
ELISA
Antibody Type
Recombinant Antibody
Immunogen
Recombinant HER2/neu.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blot using anti-HER2/neu antibody SER4 at 2ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-HER2/neu antibody SER4 at 2ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells using anti-HER2/neu antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells using anti-HER2/neu antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-HER2/neu antibody
Describes the generation of the SER4-monoclonal antibody and how the antibody inhibits cell growth in vitro.
Recombinant monoclonal antibody to HER2/neu. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SER4.
Recombinant monoclonal antibody to HER2/neu. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SER4.
Product Categories/Family for anti-HER2/neu antibody
References
Itoh et al.; Journal of Biochemistry 2003; 133(2):239-245
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
137,910 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform b
NCBI Official Synonym Full Names
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived on
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The HER2/neu erbb2 (Catalog #AAA72048) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HER2/neu [SER4], Mouse IgG1, Kappa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HER2/neu can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the HER2/neu erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HER2/neu, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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