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IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
Mouse hHR23b Monoclonal Antibody | anti-hHR23b antibody
hHR23b Mouse mAb
Gene Names
RAD23B; P58; HR23B; HHR23B
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
hHR23b, Antibody; hHR23b Mouse mAb; hHR 23b; hHR23B; HR 23B; HR23B; mHR 23B; mHR23B; P58; RAD 23B; RAD23 (S. cerevisiae) homolog B; RAD23 homolog B (S. cerevisiae); RAD23 homolog B; RAD23 yeast homolog of B; RAD23B; RD23B_HUMAN; UV excision repair protein RAD23 homolog B; XP C repair complementing complex 58 kDa; XP C repair complementing complex 58 kDa protein; XP C repair complementing protein; XP-C repair-complementing complex 58 kDa protein; XPC repair complementing complex 58 kDa; XPC repair complementing complex 58 kDa protein; XPC repair complementing protein.; anti-hHR23b antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of hHR23b and does not cross-react with related proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-hHR23b antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Purified recombinant human hHR23b protein fragments expressed in E coli.
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-hHR23b antibody (dilution 1:100).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-hHR23b antibody (dilution 1:100).)
WB (Western Blot)
(Western blot detection of hHR23b in K562.SW480.CHO-K1.3T3 and COS7 cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.)
WB (Western Blot)
(Western blot detection of hHR23b in K562.SW480.CHO-K1.3T3 and COS7 cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.)
WB (Western Blot)
(Western blot detection of hHR23b in A431.K562.Jurkat.C6.3T3 and Hela cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.)
WB (Western Blot)
(Western blot detection of hHR23b in A431.K562.Jurkat.C6.3T3 and Hela cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.)
Related Product Information for anti-hHR23b antibody
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]
Product Categories/Family for anti-hHR23b antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,171 Da
NCBI Official Full Name
UV excision repair protein RAD23 homolog B isoform 3
NCBI Official Synonym Full Names
RAD23 homolog B (S. cerevisiae)
NCBI Official Symbol
RAD23B
NCBI Official Synonym Symbols
P58; HR23B; HHR23B
NCBI Protein Information
UV excision repair protein RAD23 homolog B; RAD23, yeast homolog of, B; XP-C repair complementing protein; XP-C repair complementing complex 58 kDa; XP-C repair-complementing complex 58 kDa protein
UniProt Protein Name
UV excision repair protein RAD23 homolog B
UniProt Gene Name
RAD23B
UniProt Synonym Gene Names
HR23B; hHR23B; p58
UniProt Entry Name
RD23B_HUMAN
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Product Notes
The hHR23b rad23b (Catalog #AAA320378) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The hHR23b Mouse mAb reacts with Human, Mouse, Rat, Monkey, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's hHR23b can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the hHR23b rad23b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hHR23b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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