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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of LOVO cells with HIF1AN antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human HIF1AN Monoclonal Antibody | anti-HIF1AN antibody
HIF1AN Antibody
Gene Names
HIF1AN; FIH1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
HIF1AN, Antibody; HIF1AN Antibody; DKFZp762F1811 antibody; Factor inhibiting HIF-1 antibody; Factor inhibiting HIF1 antibody; FIH 1 antibody; FIH-1 antibody; FIH1 antibody; FLJ20615 antibody; FLJ22027 antibody; HIF1AN antibody; HIF1N_HUMAN antibody; Hypoxia inducible factor 1 alpha inhibitor antibody; Hypoxia inducible factor 1 alpha subunit inhibitor antibody; Hypoxia inducible factor asparagine hydroxylase antibody; Hypoxia-inducible factor 1-alpha inhibitor antibody; Hypoxia-inducible factor asparagine hydroxylase antibody; Peptide aspartate beta dioxygenase antibody; anti-HIF1AN antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JG37-66
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-HIF1AN antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human HIF1AN aa 1-200.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of LOVO cells with HIF1AN antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of LOVO cells with HIF1AN antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining HIF1AN in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining HIF1AN in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-HIF1AN antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-HIF1AN antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1, 000 dilution.)
Related Product Information for anti-HIF1AN antibody
Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like beta-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24.
Product Categories/Family for anti-HIF1AN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,285 Da
NCBI Official Full Name
hypoxia-inducible factor 1-alpha inhibitor
NCBI Official Synonym Full Names
hypoxia inducible factor 1, alpha subunit inhibitor
NCBI Official Symbol
HIF1AN
NCBI Official Synonym Symbols
FIH1
NCBI Protein Information
hypoxia-inducible factor 1-alpha inhibitor; FIH-1; factor inhibiting HIF-1; factor inhibiting HIF1; hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
UniProt Protein Name
Hypoxia-inducible factor 1-alpha inhibitor
UniProt Gene Name
HIF1AN
UniProt Synonym Gene Names
FIH1; FIH-1
UniProt Entry Name
HIF1N_HUMAN
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Product Notes
The HIF1AN hif1an (Catalog #AAA312667) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIF1AN Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIF1AN can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HIF1AN hif1an for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF1AN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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