Application Data
(Staining of permeablized RAW264.7 cells with Hamster anti Mouse high mobility group protein B1 followed by Goat anti Hamster IgG:PE)
Hamster HIGH MOBILITY GROUP PROTEIN B1 Monoclonal Antibody | anti-HMGB1 antibody
HAMSTER ANTI HIGH MOBILITY GROUP PROTEIN B1
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
HIGH MOBILITY GROUP PROTEIN B1, Antibody; HAMSTER ANTI HIGH MOBILITY GROUP PROTEIN B1; anti-HMGB1 antibody
Host
Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
HMG1-5H6
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
215
Applicable Applications for anti-HMGB1 antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
Recombinant mouse HMGB1
Buffer Solution
Buffer Solution
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of permeablized RAW264.7 cells with Hamster anti Mouse high mobility group protein B1 followed by Goat anti Hamster IgG:PE)
Application Data
(Staining of permeablized RAW264.7 cells with Hamster anti Mouse high mobility group protein B1 followed by Goat anti Hamster IgG:PE)
Application Data
(Immunofluorescence staining of PFA fixed permeablized NIH-3T3 cells with Hamster anti Mouse high mobility group protein B1, signal was visualized using Goat anti Hamster IgG:biotin followed by streptavidin:AlexaFluor594)
Application Data
(Immunofluorescence staining of PFA fixed permeablized NIH-3T3 cells with Hamster anti Mouse high mobility group protein B1, signal was visualized using Goat anti Hamster IgG:biotin followed by streptavidin:AlexaFluor594)
Application Data
(Western Blotting analysis of proteins and cell line lysates probed with Hamster anti Mouse high mobility group protein B1)
Application Data
(Western Blotting analysis of proteins and cell line lysates probed with Hamster anti Mouse high mobility group protein B1)
Related Product Information for anti-HMGB1 antibody
Hamster anti High Mobility Group Protein B1 antibody, clone HMG1-5H6 specifically recognizes High mobility group protein B1 (HMGB1), a ubiquitously expressed nuclear DNA binding protein, and one of a newly emerging group of alarmins, which acts as a stabilizer of nucleosome formation, and facilitates transcription factor binding, by bending DNA. HMGB1 is one of the signature danger signals of endogenous cellular injury, and is released outside the cell by necrotic and inflammatory cells to act as a chemoattractant for immature dendritic cells (DCs), promoting their maturation. DCs can also secrete HMGB1, promoting proliferation and Th1 polarization of interacting T cells. Cellular injury resulting in necrosis, leads to passive HMGB1 release, and microbes or pro-inflammatory cytokines may later stimulate active release from APCs. HMGB1 is emerging as a prime specific marker and regulator of necrotic cell death, possibly through the PI3KC3-MEK-ERK pathway. It interacts directly with the autophagy protein Beclin-1, and binds to receptors, such as RAGE on endothelial cells, and Toll-like receptors on macrophages. Studies identifying microtubule-associated protein 1 light chain 3 (LC3) lipidation and redistribution, coupled with the accumulation of autophagosomes and autolysosomes, have shown an important role for HMGB1 release in sustaining autophagy. Studies have also shown that HMGB1 released after chemotherapy treatment is a critical regulator of autophagy, and a potential drug target for therapeutic interventions in leukemia. HMGB1 is increasingly recognized as an important protein in medical research. It is angiogenic and promotes cardiac stem cell growth and differentiation, it may act as an adjuvant or assist in tissue repair, and is also a prototypical damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24 and is associated with the hallmarks of cancer. Accelerated levels of HMGB1 are seen in
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,894 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; SBP-1
NCBI Protein Information
high mobility group protein B1; Amphoterin; HMG-1; Sulfoglucuronyl carbohydrate binding protein; high mobility group protein 1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_HUMAN
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Product Notes
The HMGB1 hmgb1 (Catalog #AAA50153) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HIGH MOBILITY GROUP PROTEIN B1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the HMGB1 hmgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIGH MOBILITY GROUP PROTEIN B1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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