IP (Immunoprecipitation)
(Immunoprecipitating His in 293 whole cell lysate transfected with HisLane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His.Lane 2: 2F4B5 antibody(2ug) + 293 whole cell lysate transfected with His (500ug)Lane 3: 293 whole cell lysate transfected with His (5ug)For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)
Mouse His Monoclonal Antibody | anti-His antibody
His Monoclonal Antibody
IP (Immunoprecipitation)
(Immunoprecipitating His in 293 whole cell lysate transfected with HisLane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His.Lane 2: 2F4B5 antibody(2ug) + 293 whole cell lysate transfected with His (500ug)Lane 3: 293 whole cell lysate transfected with His (5ug)For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)
IP (Immunoprecipitation)
(Immunoprecipitating His in 293 whole cell lysate transfected with HisLane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His.Lane 2: 2F4B5 antibody(2ug) + 293 whole cell lysate transfected with His (500ug)Lane 3: 293 whole cell lysate transfected with His (5ug)For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)
WB (Western Blot)
(Western BlotPositive WB detected in:200ng of four Recombinant protein with his tagAll lanes: His antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 43.4, 62.5,56,80.6 KDaObserved band size:43.4, 66, 57 ,90 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in:200ng of four Recombinant protein with his tagAll lanes: His antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 43.4, 62.5,56,80.6 KDaObserved band size:43.4, 66, 57 ,90 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: 200ng recombinant protein with His tagHis antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64wSecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size:62.5 KDaObserved band size: 66 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: 200ng recombinant protein with His tagHis antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64wSecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size:62.5 KDaObserved band size: 66 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: 200ng recombinant protein with His tagHis antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256wSecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 43.4 KDaObserved band size: 43.4 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: 200ng recombinant protein with His tagHis antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256wSecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 43.4 KDaObserved band size: 43.4 KDaExposure time?5min)
Similar Products
Product Notes
The His (Catalog #AAA279191) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The His Monoclonal Antibody reacts with Species independent and may cross-react with other species as described in the data sheet. AAA Biotech's His can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the His for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.