IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with Histone H3 (PHH3) Recombinant Rabbit Monoclonal Antibody (PHH3/471R).)
Rabbit anti-Human Histone H3, phosphorylated (pSer10) Monoclonal Antibody | anti-H3F3 antibody
Histone H3, phosphorylated (pSer10) (Nuclear Marker) Recombinant Rabbit Monoclonal Antibody [Clone PHH3/471R]
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Histone H3, phosphorylated (pSer10), Antibody; Histone H3, phosphorylated (pSer10) (Nuclear Marker) Recombinant Rabbit Monoclonal Antibody [Clone PHH3/471R]; Histone H3, phosphorylated (pSer10) (Nuclear Marker); Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3. 1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; anti-H3F3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit/IgG
Clone Number
PHH3/471R
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-H3F3 antibody
IHC (Immunohistochemistry)
Antibody Type
Recombinant Antibody
Immunogen
A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10)
Cellular Localization
Nucleus, Chromosome
Positive Control
Human tonsil, liver or breast carcinoma.
Hu-chromosome Location
14q42.12
Unigene
132854
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with Histone H3 (PHH3) Recombinant Rabbit Monoclonal Antibody (PHH3/471R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with Histone H3 (PHH3) Recombinant Rabbit Monoclonal Antibody (PHH3/471R).)
Related Product Information for anti-H3F3 antibody
Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0. 0 to 6. 6% (median value 0. 8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0. 031), presence of tumor ulceration (p =0. 041) and tumor necrosis (p =0. 027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0. 003) and high Ki-67 expression (p = 0. 002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc. , PHH3 mAb is helpful for tumor pathological classification and prognosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,404 Da
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
NCBI Protein Information
histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
UniProt Protein Name
Histone H3.1
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
UniProt Entry Name
H31_HUMAN
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Product Notes
The H3F3 hist1h3a (Catalog #AAA216005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, phosphorylated (pSer10) (Nuclear Marker) Recombinant Rabbit Monoclonal Antibody [Clone PHH3/471R] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3, phosphorylated (pSer10) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the H3F3 hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, phosphorylated (pSer10), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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