WB (Western Blot)
(Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by Western blot. HeLa nuclear extract (20 ug) and recombinant human Histones (100 ng) were probed with Histone H3.1 / 3.2 antibody (mAb) at a 1 ug/ml dilution in lanes 1, 2, & 3. Histone H3 (mAb) is also shown at a 0.25 ug/ml dilution in lanes 4 & 5. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: 100 ng recombinant human Histone H3.1 protein. Lane 3: 100 ng recombinant human Histone H3.3 protein. Lane 4: 100 ng recombinant human Histone H3.1 protein. Lane 5: 100 ng recombinant human Histone H3.3 protein.)
Mouse Histone H3.1/3.2 Monoclonal Antibody | anti-H3.1/3.2 antibody
Histone H3.1/3.2 antibody (mAb)(Clone 1D4F2)
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
Histone H3.1/3.2, Antibody; Histone H3.1/3.2 antibody (mAb)(Clone 1D4F2); anti-H3.1/3.2 antibody
Host
Mouse
Reactivity
Human, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1D4F2
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3.1/3.2 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This antibody was raised against a peptide comprising amino acids 21-39 of human Histone H3.1. This region is 100% identical in human Histone H3.2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by Western blot. HeLa nuclear extract (20 ug) and recombinant human Histones (100 ng) were probed with Histone H3.1 / 3.2 antibody (mAb) at a 1 ug/ml dilution in lanes 1, 2, & 3. Histone H3 (mAb) is also shown at a 0.25 ug/ml dilution in lanes 4 & 5. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: 100 ng recombinant human Histone H3.1 protein. Lane 3: 100 ng recombinant human Histone H3.3 protein. Lane 4: 100 ng recombinant human Histone H3.1 protein. Lane 5: 100 ng recombinant human Histone H3.3 protein.)
WB (Western Blot)
(Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by Western blot. HeLa nuclear extract (20 ug) and recombinant human Histones (100 ng) were probed with Histone H3.1 / 3.2 antibody (mAb) at a 1 ug/ml dilution in lanes 1, 2, & 3. Histone H3 (mAb) is also shown at a 0.25 ug/ml dilution in lanes 4 & 5. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: 100 ng recombinant human Histone H3.1 protein. Lane 3: 100 ng recombinant human Histone H3.3 protein. Lane 4: 100 ng recombinant human Histone H3.1 protein. Lane 5: 100 ng recombinant human Histone H3.3 protein.)
IF (Immunofluorescence)
(Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by immunofluorescence. Top: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Bottom: Hoechst.)
IF (Immunofluorescence)
(Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by immunofluorescence. Top: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Bottom: Hoechst.)
Related Product Information for anti-H3.1/3.2 antibody
Short Description: Our Histone H3.1/3.2 antibody (mAb)(Clone 1D4F2) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.2 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.2 has a serine.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.2 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.2 has a serine.
Product Categories/Family for anti-H3.1/3.2 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
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Product Notes
The H3.1/3.2 (Catalog #AAA60062) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H3.1/3.2 antibody (mAb)(Clone 1D4F2) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3.1/3.2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H3.1/3.2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3.1/3.2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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