IHC (Immunohistochemisry)
(Immunofluorescence staining of Hela cells with AAA235168 at 1:31, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Histone H3.1 Recombinant Antibody | HIST1H3A recombinant antibody
Phospho-Histone H3.1 (S10) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
Histone H3.1, Recombinant Antibody; Phospho-Histone H3.1 (S10) Antibody; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3FA; AND; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; HIST1H3A recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity-chromatography
Form/Format
Liquid
Sequence Length
136
Applicable Applications for HIST1H3A recombinant antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
A synthesized peptide
Conjugation
Non-conjugated
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunofluorescence staining of Hela cells with AAA235168 at 1:31, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IHC (Immunohistochemisry)
(Immunofluorescence staining of Hela cells with AAA235168 at 1:31, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of AAA235168 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
ICC (Immunocytochemistry)
(Immunocytochemistry analysis of AAA235168 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysateAll lanes: Phospho-Histone H3.1 (S10)antibody at 0.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 15 KDaObserved band size: 15 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysateAll lanes: Phospho-Histone H3.1 (S10)antibody at 0.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 15 KDaObserved band size: 15 KDa)
Product Categories/Family for HIST1H3A recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,404 Da
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1 H3 family member a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
NCBI Protein Information
histone H3.1
UniProt Protein Name
Histone H3.1
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
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Product Notes
The HIST1H3A hist1h3a (Catalog #AAA235168) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Histone H3.1 (S10) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3.1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HIST1H3A hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3.1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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