DB (Dot Blot)
(Histone H3 phospho Ser10 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 mAb for phospho Ser10 histone H3. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 10. Lane 2: unmodified serine 10 peptide. Lane 3: peptide phosphorylated at serine 28. Lane 4: unmodified serine 28 peptide.)
Rabbit Histone H3S10ph Monoclonal Antibody | anti-H3S10ph antibody
Histone H3S10ph antibody (mAb)(Clone MABI 0312)
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Dot Blot
Purity
Protein G Chromatography
Synonyms
Histone H3S10ph, Antibody; Histone H3S10ph antibody (mAb)(Clone MABI 0312); anti-H3S10ph antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MABI 0312
Purity/Purification
Protein G Chromatography
Form/Format
PBS pH7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3S10ph antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot)
Conjugation
Phosphorylated
Immunogen
This Histone H3 phospho Ser10 antibody was raised against a peptide containing phospho serine 10 of human histone H3.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Histone H3 phospho Ser10 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 mAb for phospho Ser10 histone H3. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 10. Lane 2: unmodified serine 10 peptide. Lane 3: peptide phosphorylated at serine 28. Lane 4: unmodified serine 28 peptide.)
DB (Dot Blot)
(Histone H3 phospho Ser10 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 mAb for phospho Ser10 histone H3. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 10. Lane 2: unmodified serine 10 peptide. Lane 3: peptide phosphorylated at serine 28. Lane 4: unmodified serine 28 peptide.)
WB (Western Blot)
(Histone H3S10ph antibody (mAb) tested by Western blot. HeLa nuclear extract (30 ug per lane) was probed with Histone H3S10ph antibody at a 2 ug/ml dilution. Lane 1: No treatment. Lane 2: Cells treated with colcemid.)
WB (Western Blot)
(Histone H3S10ph antibody (mAb) tested by Western blot. HeLa nuclear extract (30 ug per lane) was probed with Histone H3S10ph antibody at a 2 ug/ml dilution. Lane 1: No treatment. Lane 2: Cells treated with colcemid.)
IF (Immunofluorescence)
(Histone H3 phospho Ser10 mAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 phospho Ser10 mAb (1 ug/ml, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
IF (Immunofluorescence)
(Histone H3 phospho Ser10 mAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 phospho Ser10 mAb (1 ug/ml, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
Related Product Information for anti-H3S10ph antibody
Short Description: Our Histone H3S10ph antibody (mAb)(Clone MABI 0312) was raised in a Mouse host. It has been validated for use in Dot blot, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.
Product Categories/Family for anti-H3S10ph antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3S10ph (Catalog #AAA59902) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3S10ph antibody (mAb)(Clone MABI 0312) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3S10ph can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot). Researchers should empirically determine the suitability of the H3S10ph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3S10ph, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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